Leishmania (Viannia) braziliensis genotypes identified in lesions of patients with atypical or typical manifestations of tegumentary leishmaniasis: Evaluation by two molecular markers
Leishmania (Viannia) braziliensis genotypes identified in lesions of patients with atypical or typical manifestations of tegumentary leishmaniasis: Evaluation by two molecular markers
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DOI：
10.1016/j.exppara.2008.12.006
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发表时间：
2009-04-01
影响因子：
2.1
通讯作者：
Pacheco, R. S.
中科院分区：
文献类型：
--
作者：
Baptista, C.;Schubach, A. O.;Pacheco, R. S.
使用 MLEE、RAPD 和 LSSP-PCR 分析来比较从具有罕见临床表现的患者病变和典型病变患者病变中获得的美洲外皮利什曼病 (ATL) 分离株。 MLEE 分析的所有 34 个样品都显示出与巴西利什曼原虫 (Viannia) 参考菌株相似的电形态特征。通过 RAPD 分析，确定了九种遗传图谱（基因型）。 LSSP-PCR 证实了初步筛选，表型分析将分离株分为两大类，包括九种不同的基因型。在最大数量的分离株中检测到了定义为 LbmtDNAgen1 的流行基因型。基因型和临床症状之间没有关联。然而，在同一患者的初始病变 (LbmtDNAGen9) 和重新激活病变 (LbmtDNAGen3) 中可以鉴定出两种不同的基因型。我们的结果支持了里约热内卢州 L (V.) braziliensis 中基因型多样性不太明显的观点，并证明了这些分子标记在遗传变异研究中的有用应用。 (C) 2008 Elsevier Inc. 保留所有权利。
Analyses of MLEE, RAPD and LSSP-PCR were used to compare the panel of american tegumentary leishmaniasis (ATL) isolates obtained from lesions of patients with rare clinical manifestations of the disease and typical lesions. All of the 34 samples analyzed by MLEE demonstrated similar electromorphic profiles with Leishmania (Viannia) braziliensis reference strain. Through the RAPD analysis, nine genetic profiles (genotypes) were identified. LSSP-PCR corroborates the initial screening and phenetic analysis has grouped the isolates into two major clusters comprising the nine different genotypes. Prevalent genotype defined as LbmtDNAgen1 was detected in the largest number of isolates. There was no association between genotypes and clinical symptoms. However, two different genotypes could be identified in the initial (LbmtDNAGen9) and reactivated lesion (LbmtDNAGen3) of the same patient. Our results support the idea of a less pronounced genotypic diversity among L (V.) braziliensis circulating in the State of Rio de Janeiro and demonstrate the useful application of these molecular markers in genetics variability studies. (C) 2008 Elsevier Inc. All rights reserved.