THE PHYTOPATHOGENIC FUNGUS CLADOSPORIUM-FULVUM IS NOT SENSITIVE TO THE CHITINASE AND BETA-1,3-GLUCANASE DEFENSE PROTEINS OF ITS HOST, TOMATO
THE PHYTOPATHOGENIC FUNGUS CLADOSPORIUM-FULVUM IS NOT SENSITIVE TO THE CHITINASE AND BETA-1,3-GLUCANASE DEFENSE PROTEINS OF ITS HOST, TOMATO
复制标题
DOI：
10.1006/pmpp.1995.1004
复制
发表时间：
1995-01-01
影响因子：
2.7
通讯作者：
DEWIT, PJGM
中科院分区：
文献类型：
--
作者：
JOOSTEN, MHAJ;VERBAKEL, HM;DEWIT, PJGM
番茄（Lycopersicon esculentum）接种黄枝孢菌（Cladosporium fulvum）的无毒小种导致宿主中胞外和胞内几丁质酶和β-1，3-葡聚糖酶的显著增加。本文描述了从C.番茄叶腐病从这样的叶子，含有一套完整的额外的和细胞内的番茄几丁质酶和β-1，3-葡聚糖酶，通过体外生物测定的粗蛋白制剂的抗真菌活性的评估，揭示了Lest真菌绿色木霉是高度敏感的水解酶。发现抗真菌活性存在于几丁质酶和β-1，S-葡聚糖酶的基本细胞内同种型中。酸性胞外酶没有显示出任何抗真菌活性。几丁质酶和β-1，3-葡聚糖酶粗品对番茄褐腐病菌分生孢子的萌发没有抑制作用，即使在高浓度下，这些粗品对番茄褐腐病菌也没有抑菌活性。番茄几丁质酶和β-1，S-葡聚糖酶在番茄抗病性中的作用。fulvum，讨论。
Inoculation of tomato (Lycopersicon esculentum) with an avirulent race of Cladosporium fulvum results in a substantial increase in extra- and intracellular chitinases and beta-1,3-glucanases in the host. In this communication we describe the purification of a 27 kDa, extracellular chitinase [isoelectric point (pI) < 4.8] and two intracellular chitinases (30 and 32 kDa, pI 9.1 and pI > 10, respectively), from C. fulvum-infected tomato leaves. Assessment of the antifungal activity of crude protein preparations from such leaves, containing a complete set of the extra- and intracellular tomato chitinases and beta-1,3-glucanases, by in vitro bioassays, revealed that the Lest Fungus Trichoderma viride is highly sensitive to the hydrolytic enzymes. The antifungal activity was found to reside in the basic, intracellular isoforms of the chitinases and beta-1,S-glucanases. The acidic, extracellular enzymes did not show any antifungal activity. Germinated conidia of C.fulvum were not inhibited by crude preparations of chitinase and beta-1,3-glucanase; even at high concentrations these preparations showed no antifungal activity against C.fulvum.The apparent insensitivity of C. fulvum to tomato chitinases and beta-1,S-glucanases in in vitro bioassays and the role of these enzymes in resistance of tomato against C. fulvum, are discussed.