Silicon‐mediated multiple interactions: Simultaneous induction of rice defense and inhibition of larval performance and insecticide tolerance of. Chilo suppressalis. by sodium silicate
Silicon‐mediated multiple interactions: Simultaneous induction of rice defense and inhibition of larval performance and insecticide tolerance of. Chilo suppressalis. by sodium silicate
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硅介导的多重相互作用：同时诱导水稻防御并抑制幼虫性能和杀虫剂耐受性。
DOI：
10.1002/ece3.6235
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发表时间：
2020
影响因子：
2.6
通讯作者：
Yuanyuan Song
中科院分区：
文献类型：
--
作者：
Jie Wang;Rongrong Xue;Xueyang Ju;Hui Yan;Zhou Gao;Mohammed Esmail Abdalla Elzaki;Lin Hu;Rensen Zeng;Yuanyuan Song
二化螟是危害水稻生产的重要害虫之一。硅介导的水稻对各种害虫的抗性已被广泛报道，硅酸钠(SS)已被用作植物应用的有效硅来源。然而，关于施硅对草食性昆虫的直接影响的信息相当有限。用含0.5和2.0 mg/L SS的营养液培养的水稻植株和含0.1%和0.5%SS的人工饲料饲养SSB幼虫，测定其对杀虫剂的抗性。无论是在水稻培养液中还是在人工饲料中添加SS，都显著抑制了中肠乙酰胆碱酯酶、谷胱甘肽S转移酶的活性和细胞色素P450蛋白的水平。取食含SS饲料的幼虫对杀虫剂的耐受性较低。此外，RNA-seq分析表明，SS介导的幼虫对杀虫剂的耐受性与脂肪酸生物合成和丙酮酸代谢途径密切相关。我们的结果表明，硅不仅提高了植物对昆虫食草动物的抗性，而且还削弱了昆虫对杀虫剂的解毒能力。这应该被认为是硅介导的植物-昆虫相互作用的另一个重要方面，并可能为害虫管理提供一种新的途径。
The rice striped stem borer (SSB,Chilo suppressalis) is one of the most destructive pests of rice plants. Si‐mediated rice defense against various pests has been widely reported, and sodium silicate (SS) has been used as an effective source of silicon for application to plants. However, there is quite limited information about the direct effects of Si application on herbivorous insects. SSB larval performance and their insecticide tolerance were examined after they had been reared either on rice plants cultivated in nutrient solution containing 0.5 and 2.0 mM SS or on artificial diets with 0.1% and 0.5% SS. SS amendment in either rice culture medium or artificial diets significantly suppressed the enzymatic activities of acetylcholinesterase, glutathione S‐transferases, and levels of cytochrome P450 protein in the midgut ofC. suppressalislarvae. Larvae fed on diets containing SS showed lower insecticide tolerance. Additionally, RNA‐seq analysis showed that SS‐mediated larval insecticide tolerance was closely associated with fatty acid biosynthesis and pyruvate metabolism pathways. Our results suggest that Si not only enhances plant resistance against insect herbivore, but also impairs the insect's capacity to detoxify the insecticides. This should be considered as another important aspect in Si‐mediated plant–insect interaction and may provide a novel approach of pest management.