Comparative evaluation of intestinal nitric oxide in embryonic zebrafish exposed to metal oxide nanoparticles.
Comparative evaluation of intestinal nitric oxide in embryonic zebrafish exposed to metal oxide nanoparticles.
复制标题
DOI：
10.1002/smll.201301087
复制
发表时间：
2013-12
期刊：
影响因子：
13.3
通讯作者：
Rıfat Emrah Ozel;Ramiz S. J. Alkasir;Kayla Ray;Kenneth N Wallace;S. Andreescu
中科院分区：
文献类型：
--
作者：
Rıfat Emrah Ozel;Ramiz S. J. Alkasir;Kayla Ray;Kenneth N Wallace;S. Andreescu
纳米颗粒（NP）暴露可通过产生活性氧和氮物质诱导氧化应激，这可导致细胞和组织损伤。消化系统是受NP暴露影响的最初器官之一。在这里，它表明，暴露于金属氧化物纳米颗粒诱导斑马鱼肠道NO浓度的差异变化。肠NO浓度用插入活胚胎肠中的碳纤维微电极电化学定量。通过NO特异性药理学操作证明电化学信号的特异性，并且结果与4，5-二氨基荧光素-二乙酸酯（DAF-FM-DA）相关。研究表明，纳米颗粒可以诱导或降低生理NO水平，具体取决于其氧化还原反应性、类型和剂量。斑马鱼胚胎暴露于不同阶段和浓度的CuO和CeO 2纳米颗粒后，NO水平发生变化。CuO纳米颗粒增加NO浓度，表明肠道氧化损伤。相反，低CeO 2 NP浓度暴露显著降低NO水平，表明NO清除活性。然而，高浓度暴露导致NO增加。NO浓度的变化表明肠道生理学和氧化应激的变化，这最终将对应于NP毒性。这项工作还表明，使用电化学监测斑马鱼器官内的NO在体内的变化。
Nanoparticle (NP) exposure may induce oxidative stress through generation of reactive oxygen and nitrogen species, which can lead to cellular and tissue damage. The digestive system is one of the initial organs affected by NP exposure. Here, it is demonstrated that exposure to metal oxide NPs induces differential changes in zebrafish intestinal NO concentrations. Intestinal NO concentrations are quantified electrochemically with a carbon fiber microelectrode inserted in the intestine of live embryos. Specificity of the electrochemical signals is demonstrated by NO-specific pharmacological manipulations and the results are correlated with the 4,5-diaminofluorescein-diacetate (DAF-FM-DA). NPs are demonstrated to either induce or reduce physiological NO levels depending on their redox reactivity, type and dose. NO level is altered following exposure of zebrafish embryos to CuO and CeO2 NPs at various stages and concentrations. CuO NPs increase NO concentration, suggesting an intestinal oxidative damage. In contrast, low CeO2 NP concentration exposure significantly reduces NO levels, suggesting NO scavenging activity. However, high concentration exposure results in increased NO. Alterations in NO concentration suggest changes in intestinal physiology and oxidative stress, which will ultimately correspond to NPs toxicity. This work also demonstrates the use of electrochemistry to monitor in vivo changes of NO within zebrafish organs.