MONOCLONAL-ANTIBODY DEFINING TAX1 PROTEIN OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I
MONOCLONAL-ANTIBODY DEFINING TAX1 PROTEIN OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I
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DOI：
10.1620/tjem.157.1
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发表时间：
1989-01-01
影响因子：
2.2
通讯作者：
TOZAWA, H
中科院分区：
文献类型：
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作者：
LEE, B;TANAKA, Y;TOZAWA, H
我们制备了一种单克隆抗体（MAb），Lt-4，它识别在HTLV-I感染的细胞中表达的tax 1蛋白。间接免疫荧光染色显示，Lt-MAb识别的抗原主要定位于HUT 102、TCL-As 2、MT-1和MT-2等细胞核内，MT-2细胞胞浆内也有少量定位。Lt-4单克隆抗体不与两个HTLV-I未感染的T细胞系测试和新鲜和PHA激活的外周血淋巴细胞（PBL）的几个正常供体。此外，Lt-4单克隆抗体染色的HeLa细胞的细胞核感染的重组牛痘病毒编码的tax 1，但不与野生型牛痘病毒。在Western blot（WB）和放射免疫沉淀分析中，Lt-4 MAb在HUT 102和TCL-As 2细胞中检测到40 kDa分子（p40），在MT-2细胞中检测到p40和p68。这些结果表明，Lt-4单克隆抗体是高度特异性的tax 1蛋白。
We prepared a monoclonal antibody (MAb), Lt-4, which recognizes a tax1 protein expressed in HTLV-I-infected cells. Indirect immunofluorescence staining showed that the antigen recognized by Lt-MAb located mainly in the nuclei of HTLV-I-infected T cell lines such as HUT102, TCL-As2, MT-1 and MT-2, and also weakly in the cytoplasm of MT-2 cell line. Lt-4 MAb did not react with two HTLV-I-uninfected T cell lines tested and fresh and PHA-activated peripheral blood lymphocytes (PBL) of several normal donors. Furthermore, Lt-4 MAb stained the nuclei of HeLa cells infected with a recombinant vaccinia virus encoding the tax1 but not with a wild type vaccinia virus. In Western blot (WB) and radioimmunoprecipitation analyses, Lt-4 MAb detected a 40 kDa molecule (p40) in HUT102 and TCL-As2 cells, and p40 and p68 in MT-2 cells. These results show that Lt-4 MAb is highly specific for the tax1 protein.