Riboregulator elements as tools to engineer gene expression in cyanobacteria
Riboregulator elements as tools to engineer gene expression in cyanobacteria
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DOI：
10.1007/s00253-018-9221-0
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发表时间：
2018-07
影响因子：
5
通讯作者：
Kinuko Ueno;K. Tsukakoshi;K. Ikebukuro
中科院分区：
文献类型：
--
作者：
Kinuko Ueno;K. Tsukakoshi;K. Ikebukuro
蓝藻是生产生物燃料的理想宿主。虽然已经努力通过基因工程改造蓝藻以高效地生产生物燃料和其他重要化学品，但可以应用于蓝藻的工具仍然有限。一种新的基因调控工具--核糖体调节器已经在蓝藻中得到应用。核调节因子是一种受自然启发的RNA工具，由两个人工设计的RNA片段组成。由于其高度的特异性和有效性，适合在蓝藻中进行代谢工程，人们已经进行了一些研究来优化和改进核转录调节因子的功能。在这篇综述中，我们重点介绍了最近对核调节因子的改进，并将它们与其他在蓝藻中开发的RNA介导的基因调控工具进行比较，以探索核调节因子的未来应用。
Cyanobacteria are an ideal host for biofuel production. Although efforts have been made to genetically engineer cyanobacteria for efficient production of biofuels and other important chemicals, the tools that can be applied to cyanobacteria are still limited. A new gene regulation tool, riboregulator, has been examined for application in cyanobacteria. A riboregulator is a nature-inspired RNA tool, which is composed of two artificially designed RNA fragments. Owing to its high specificity and efficacy, it is suitable for metabolic engineering in cyanobacteria, and several studies have been done to optimize and improve the function of the riboregulator. In this review, we focus on the recent improvements made to riboregulators and compare them with other RNA-mediated gene regulation tools developed in cyanobacteria to investigate future applications of riboregulators.