BIOLOGICAL AND PHYSICAL PROPERTIES OF THE RYAN STRAIN OF FILAMENTOUS INFLUENZA VIRUS
BIOLOGICAL AND PHYSICAL PROPERTIES OF THE RYAN STRAIN OF FILAMENTOUS INFLUENZA VIRUS
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DOI：
10.1099/00221287-19-1-23
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发表时间：
1958-01-01
期刊：
影响因子：
--
通讯作者：
ABBOT, A
中科院分区：
文献类型：
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作者：
ADA, GL;PERRY, BT;ABBOT, A
1954年在墨尔本分离出的A型流感病毒Ryan含有约20%的颗粒状纤维。利用电子显微镜进行颗粒计数，估计Ryan“长”丝（长：宽bbbb6的颗粒）作为血凝素的效率约为pr8病毒球体的6倍。Ryan病毒的尿囊液制剂具有较高的比值（log） EID 50:AD值，并且估计表明，最多6“长”丝相当于一个EID 50，而PR 8病毒为18个颗粒，Ryan病毒突变株为14个颗粒，几乎完全以球体的形式存在。与病毒pr8球（16粒：AD）相比，丝状Ryan制剂中的球形颗粒作为血凝素的效率较低（29 ~ 38粒：AD）。这些颗粒在“长”细丝形成之前，在收获的液体中形成21小时。接种后“长”丝比例很低时，约9个球形颗粒构成1 EID 50。为了进行后续的化学检查，通过对红细胞进行特异性吸附和洗脱的过程分离和浓缩细丝，然后进行3次差速离心。在这种制剂中，丝与球的比例约为50:50。“纯化”细丝的重量约为球形颗粒的30倍，占纯化制剂中总重量的95%以上。
An influenza A strain, Ryan, isolation in Melbourne in 1954 contains on a particle basis about 20% filaments. By using the electron microscope for particle counting, the efficiency as haemagglutinin of Ryan "long" filaments (particles in which length:width >6) was estimated to be about 6 times greater than that of spheres of PR 8 virus. Allantoic fluid preparations of Ryan virus have high values for the ratio (log) EID 50:AD and estimates indicate that a maximum of 6 "long" filaments are equivalent to one EID 50, compared with 18 particles in the case of PR 8 virus or 14 particles in the case of a mutant strain of Ryan virus which exists almost exclusively as spheres. Compared with virus PR 8 spheres (16 particles: AD), spherical particles present in filamentous Ryan preparations have a low efficiency as haemagglutinin (29 to 38 particles: AD). These particles are formed before the "long" filaments, and in fluids harvested 21 hr. after inoculation when the proportion of "long" filaments is very low, about 9 spherical particles constitute 1 EID 50. For the purpose of subsequent chemical examinations filaments were isolated and concentrated by a process involving specific adsorption to and elution from red cells, followed by 3 cycles of differential centrifugation. The ratio of filaments: spheres in such preparations is about 50:50. "Purified" filaments weigh about 30 times as much as the spherical particles present and account for over 95% of the total weight present in purified preparations.