Transcriptional regulation of connective tissue growth factor by sphingosine 1-phosphate in rat cultured mesangial cells
Transcriptional regulation of connective tissue growth factor by sphingosine 1-phosphate in rat cultured mesangial cells
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DOI：
10.1016/j.febslet.2005.03.073
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发表时间：
2005-05-09
期刊：
影响因子：
3.5
通讯作者：
Tsujimoto, G
中科院分区：
文献类型：
--
作者：
Katsuma, S;Ruike, Y;Tsujimoto, G
结缔组织生长因子（CTGF）是由转化生长因子-β（TGF-β）通过Smad活化在系膜细胞中诱导的。我们最近报道了1-磷酸鞘氨醇（SIP）诱导大鼠系膜细胞CTGF表达。然而，SIP诱导CTGF表达的机制尚不清楚。本研究揭示SIP诱导的CTGF表达是通过百日咳毒素不敏感途径介导的，其参与Rho家族的小GTP酶和蛋白激酶C的激活。我们还通过荧光素酶报告基因分析和染色质免疫沉淀显示，SIP通过Smad激活诱导CTGF表达，就像TGF-β一样。(c)2005年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Connective tissue growth factor (CTGF) is induced by transforming growth factor-beta (TGF-beta) via Smad activation in mesangial cells. We recently reported that sphingosine 1-phos- phate (SIP) induces CTGF expression in rat cultured mesangial cells. However, the mechanism by which SIP induces CTGF expression is unknown. The present study revealed that SIP-induced CTGF expression is mediated via pertussis toxin-insensitive pathways, which are involved in the activation of small GTPases of the Rho family and protein kinase C. We also showed by luciferase reporter assays and chromatin immunoprecipitation that SIP induces CTGF expression via Smad activation as TGF-beta does. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.