Detection and Collection System of Target Single Cell Based on pH and Oxygen Sensing
Detection and Collection System of Target Single Cell Based on pH and Oxygen Sensing
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DOI：
10.20965/jrm.2010.p0639
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发表时间：
2010-10-01
影响因子：
1.1
通讯作者：
Iribe, Yasunori
中科院分区：
文献类型：
--
作者：
Suzuki, Masayasu;Tanaka, Hiroyuki;Iribe, Yasunori
本文描述了基于单细胞的检测和收集，使用 pH 和氧气传感以及我们之前开发的微阵列化学传感器来并行监测单细胞活动。这种传感器由用于 pH 值或氧气的光学传感器薄膜和用炭黑掺杂的聚二甲基硅氧烷 (PDMS) 制备的微孔阵列组成。我们使用微阵列氧传感器并行监测单细胞呼吸。我们开发的自动单细胞收集器可与商用倒置显微镜一起使用。我们基于呼吸或代谢活动开发的基于单细胞的检测和收集结合了这两种技术。基于代谢活性的单细胞检测和收集的模型实验使用脲酶固定微珠（6 μ m i.d.）。注射底物后荧光强度仅在含有脲酶固定微珠的孔中增加。只需按下控制器按钮，即可成功单独收集目标孔中的珠子，而将其他珠子留在其孔中，无需任何培训或技能。
This paper describes single-cell-based detection and collection using pH and oxygen sensing with microarrayed chemical sensors we developed previously to monitor single-cell activity in parallel. Such sensors consist of optical sensor film for pH or oxygen and microwell arrays prepared with carbon-black-doped polydimethylsiloxane (PDMS). We monitored single-cell respiration in parallel using a microarrayed oxygen sensor. An automatic single-cell collector we developed can be used with a commercial inverted microscope. The single-cell-based detection and collection we developed based on respiration or metabolic activity combines these two techniques. Model experiments for single-cell-based detection and collection based on metabolic activity used urease-immobilized microbeads (6 mu m i.d.). Fluorescence intensity after substrate injection increased only in wells containing urease-immobilized microbeads. Bead in target wells could be successfully collected alone, leaving other beads in their wells, by simply pushing a controller button, requiring no training or skill.