Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology
Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology
复制标题
DOI：
10.1101/cshperspect.a023812
复制
发表时间：
2017-01-01
影响因子：
7.2
通讯作者：
Ellington, Andrew D.
中科院分区：
文献类型：
--
作者：
Hughes, Randall A.;Ellington, Andrew D.
DNA寡核苷酸的化学合成及其通过基因合成方法组装成合成子、基因、电路甚至整个基因组，已成为现代分子生物学的一项使能技术，并使设计、构建、测试、学习和重复循环成为合成生物学创新的基础。从这个角度来看，我们简要回顾了能够合成DNA寡核苷酸及其组装成更大DNA结构的技术和技术，并重点介绍了最近在降低合成成本和提高序列保真度方面的进展。开发低成本的方法来生产高质量的合成DNA，将通过降低使用成本来探索更大的生物学假设，并有助于缩小DNA读写成本差距。
The chemical synthesis of DNA oligonucleotides and their assembly into synthons, genes, circuits, and even entire genomes by gene synthesis methods has become an enabling technology for modern molecular biology and enables the design, build, test, learn, and repeat cycle underpinning innovations in synthetic biology. In this perspective, we briefly review the techniques and technologies that enable the synthesis of DNA oligonucleotides and their assembly into larger DNA constructs with a focus on recent advancements that have sought to reduce synthesis cost and increase sequence fidelity. The development of lower-cost methods to produce high-quality synthetic DNA will allow for the exploration of larger biological hypotheses by lowering the cost of use and help to close the DNA read-write cost gap.