Male-specific sterility caused by the loss of CR16
Male-specific sterility caused by the loss of CR16
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DOI：
10.1111/j.1365-2443.2007.01088.x
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发表时间：
2007-06-01
期刊：
影响因子：
2.1
通讯作者：
Takenawa, Tadaomi
中科院分区：
文献类型：
--
作者：
Suetsugu, Shiro;Banzai, Yoshibumi;Takenawa, Tadaomi
编码被称为“皮质类固醇和区域表达16”（CR 16）的蛋白质的基因已被证明受糖皮质激素调节。CR 16是Wiskott-Aldrich综合征蛋白（WASP）相互作用蛋白（WASP）家族的成员。它与神经WASP（N-WASP）结合，激活Arp 2/3复合物以诱导肌动蛋白聚合。CR 16在睾丸中高度表达，特别是在支持细胞中，支持细胞具有精子祖细胞，在精子发生中起重要作用。我们在CR 16基因敲除小鼠中发现了雄性特异性不育。CR 16基因敲除小鼠的精子头部形态异常，体外受精实验中受精能力大大降低。CR 16和N-WASP定位于支持细胞-精子细胞连接处的肌动蛋白丝。在CR 16基因敲除小鼠的睾丸和Sertoli细胞中，N-WASP的水平下降，但转录物没有下降。因此，CR 16和N-WASP可能在精子发生中起重要作用。
The gene encoding the protein known as "corticosteroids and regional expression 16" (CR16) has been shown to be regulated by glucocorticoids. CR16 is a member of the Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP) family. It binds to the neural WASP (N-WASP), which activates the Arp2/3 complex to induce actin polymerization. CR16 is highly expressed in the testes, particularly in the Sertoli cells, which harbor sperm progenitors and play an important role in spermatogenesis. We found male-specific sterility in the CR16-knockout mice. The sperms of the CR16-knockout mice had abnormal head morphology, and greatly diminished fertilization ability in in vitro fertilization experiments. CR16 and N-WASP were localized to the actin filaments at the Sertoli cell-spermatid junctions (SspJs). The level of N-WASP but not the transcript was decreased in the testes and Sertoli cells of the CR16-knockout mice. Therefore, CR16 and N-WASP are suggested to play important roles in spermatogenesis.