Density-dependent cooperative non-specific binding in solid-phase SELEX affinity selection.
Density-dependent cooperative non-specific binding in solid-phase SELEX affinity selection.
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DOI：
10.1093/nar/gkt477
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发表时间：
2013-08
影响因子：
14.9
通讯作者：
Shalloway D
中科院分区：
文献类型：
--
作者：
Ozer A;White BS;Lis JT;Shalloway D
The non-specific binding of undesired ligands to a target is the primary factor limiting the enrichment of tight-binding ligands in affinity selection. Solution-phase non-specific affinity is determined by the free-energy of ligand binding to a single target. However, the solid-phase affinity might be higher if a ligand bound concurrently to multiple adjacent immobilized targets in a cooperative manner. Cooperativity could emerge in this case as a simple consequence of the relationship between the free energy of binding, localization entropy and the spatial distribution of the immobilized targets. We tested this hypothesis using a SELEX experimental design and found that non-specific RNA aptamer ligands can concurrently bind up to four bead-immobilized peptide targets, and that this can increase their effective binding affinity by two orders-of-magnitude. Binding curves were quantitatively explained by a new statistical mechanical model of density-dependent cooperative binding, which relates cooperative binding to both the target concentration and the target surface density on the immobilizing substrate. Target immobilization plays a key role in SELEX and other ligand enrichment methods, particularly in new multiplexed microfluidic purification devices, and these results have strong implications for optimizing their performance.
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DOI：
10.1126/science.1207339
发表时间：
2011-07-29
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Paige JS;Wu KY;Jaffrey SR
通讯作者：
Jaffrey SR
影响因子：
3
作者：
Jung, Hyunsook;Robison, Aaron D.;Cremer, Paul S.
通讯作者：
Cremer, Paul S.
影响因子：
5.6
作者：
IRVINE, D;TUERK, C;GOLD, L
通讯作者：
GOLD, L
影响因子：
3.4
作者：
Zhao, Hongxia;Hakala, Markku;Lappalainen, Pekka
通讯作者：
Lappalainen, Pekka
DOI：
10.1073/pnas.1009331107
发表时间：
2010-08-31
影响因子：
11.1
作者：
Cho, Minseon;Xiao, Yi;Soh, H. Tom
通讯作者：
Soh, H. Tom