Impaired endothelial progenitor cell mobilization and colony-forming capacity in chronic obstructive pulmonary disease
Impaired endothelial progenitor cell mobilization and colony-forming capacity in chronic obstructive pulmonary disease
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DOI：
10.1111/j.1440-1843.2011.01959.x
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发表时间：
2011-05-01
期刊：
影响因子：
6.9
通讯作者：
Kato, Masato
中科院分区：
文献类型：
--
作者：
Takahashi, Toru;Suzuki, Satoshi;Kato, Masato
背景和目的：最近的研究表明，COPD患者的体循环和肺循环都存在内皮损伤。内皮祖细胞（EPC）通过生理应激因素（如手术）动员进入循环，并被认为在受损内皮的修复中发挥作用。由于并发症和肺癌的发生率，COPD患者的手术频率稳步增加;然而，尚未检查肺切除术期间EPC的动员情况。方法：采用流式细胞术检测COPD患者（n = 30）和非COPD患者（n = 30）行开胸手术患者外周血中EPC（CD 34/KDR/AC 133阳性单核细胞）的数量。结果：非COPD患者术后2 h循环EPC和EPC-CFU均显著升高，而COPD患者术后2 h循环EPC和EPC-CFU无明显变化。多元线性回归分析表明，COPD的存在是唯一显着的独立预测因素减少动员的EPC在胸部surgery.Conclusions：循环EPC和EPC-CFU的数量并没有增加，在COPD患者胸部手术。这些结果表明，在COPD患者中EPC的动员和增殖能力均严重受损。
Background and objective: Recent studies suggest that there is endothelial impairment in both the systemic and pulmonary circulations of patients with COPD. Endothelial progenitor cells (EPC) are mobilized into the circulation by physiological stressors such as surgery, and are thought to play a role in the repair of damaged endothelium. There has been a steady increase in the frequency of surgery among COPD patients, due to the incidence of complications and lung cancer; however, the mobilization of EPC during lung resection has not been examined. We evaluated whether the mobilization and proliferation of EPC are impaired in COPD patients.Methods: The numbers of circulating EPC (CD34/KDR/AC133-positive mononuclear cells) were measured by flow cytometry, in COPD patients (n = 30) and non-COPD patients (n = 30) who were undergoing thoracic surgery. EPC colony-forming units (EPC-CFU) were also examined.Results: In non-COPD patients, both circulating EPC and EPC-CFU were significantly increased 2 h after the operation started, whereas in COPD patients there were no changes in circulating EPC or EPC-CFU, irrespective of the severity of COPD. Multiple linear regression analysis demonstrated that the presence of COPD was the only significant independent predictor of reduced mobilization of EPC during thoracic surgery.Conclusions: The number of circulating EPC and EPC-CFU was not increased during thoracic surgery in COPD patients. These results indicate that both the mobilization and proliferative capacity of EPC are severely impaired in COPD patients.