DNA electrotransfer:: its principles and an updated review of its therapeutic applications
DNA electrotransfer:: its principles and an updated review of its therapeutic applications
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DOI：
10.1038/sj.gt.3302367
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发表时间：
2004-10-01
期刊：
影响因子：
5.1
通讯作者：
Mir, LM
中科院分区：
文献类型：
--
作者：
André, F;Mir, LM
使用电脉冲来转染所有类型的细胞是众所周知的，并且在体外常规用于细菌和真核细胞转化。电脉冲也可以通过经皮或与组织直接接触的电极在体内递送。在组织中注射裸DNA后，适当的局部电脉冲可以导致转移基因的非常高的表达。这份手稿描述了概念的演变和各种优化步骤，这些步骤导致使用适合DNA电转移中电脉冲的已知作用的脉冲组合，即细胞电透化和DNA电泳。还报告了迄今为止发表的主要应用的总结，仅限于使用治疗基因的体内临床前试验。
The use of electric pulses to transfect all types of cells is well known and regularly used in vitro for bacteria and eukaryotic cells transformation. Electric pulses can also be delivered in vivo either transcutaneously or with electrodes in direct contact with the tissues. After injection of naked DNA in a tissue, appropriate local electric pulses can result in a very high expression of the transferred genes. This manuscript describes the evolution in the concepts and the various optimization steps that have led to the use of combinations of pulses that fit with the known roles of the electric pulses in DNA electrotransfer, namely cell electropermeabilization and DNA electrophoresis. A summary of the main applications published until now is also reported, restricted to the in vivo preclinical trials using therapeutic genes.