Teratologic evaluation ofp-Dichlorobenzene in the rat
Teratologic evaluation ofp-Dichlorobenzene in the rat
复制标题
对二氯苯对大鼠的致畸评价
DOI：
10.1007/bf01607744
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发表时间：
1986
影响因子：
2.7
通讯作者：
C. Vismara
中科院分区：
文献类型：
--
作者：
E. Giavini;M. L. Broccia;M. Prati;C. Vismara
材料和方法体重190±20g的意大利查尔斯河处女大鼠，经过两周的驯化，用RRA关在笼子里过夜。在受控环境(光照/黑暗12小时，温度22±2摄氏度，相对湿度60%，食物和水随意)中饲养相同品系的真雄鸟(2只雌性：L雄性)。交配是通过检测下一次分娩(怀孕第一天)附近阴道内的精子来确认的。将怀孕的雌性动物单独关在笼子里，分为五组(Tab.于妊娠第6~15天分别给予0、250、500、750或1000~/kg/d的f；DCB(99%纯度，瑞士福尔卡)，等量灌胃(2.5ml/kg)。每天观察雌鼠的临床毒性体征；于妊娠第1、6、9、12、15、18和21天测定食量并记录体重，最后第21天处死动物进行尸检，观察主要脏器相关病理征象。取下肝脏，称重。子宫暴露在外面，
MATERIAlS AND METHODSVirgin CD-rats (Charles River, Calco, Italy) weighing 190+ 20g, after two weeks of acclimatization, were caged overnight with rra. ture males of the same strain (2 females: l male) in a controlled environment (12 hours light/darkness, temperature 22+ 2oc, 60% relative hLDnidity, and food and water ad libitum). Mating was confirmed by detection of spermatozoa in the vaginal snear the following rorning (day 1 of gestation). Pregnant females were caged individually, divided into five groups (Tab. l) and treated on days 6 through 15 of gestation with 0, 250, 500, 750 or 1000~/kg/day of f; DCB (99% purity, Fluka, switzerland) dissolved in corn oil and administered by gavage at constant volume (2.5 ml/kg).All females were observed daily for possible clinical signs of toxicity; the food consumption was determined daily and the body weight was recorded on days 1, 6, 9, 12, 15, 18 and 21 of pregnancy, on the last of which, day 21, they were sacrificed to undergo necroscopy to identify major organ related pathological signs. The livers were removed and weighed. The uteri were exposed and