Collagen-Induced Arthritis in Mice
Collagen-Induced Arthritis in Mice
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DOI：
10.1007/978-1-60761-058-8_11
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发表时间：
2010-01-01
期刊：
影响因子：
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通讯作者：
Tak, Paul P.
中科院分区：
文献类型：
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作者：
Bevaart, Lisette;Vervoordeldonk, Margriet J.;Tak, Paul P.
小鼠中胶原诱导的关节炎（CIA）是类风湿性关节炎（RA）的动物模型，并且可以使用不同的方案在DBA/1和C57 BL/6小鼠中诱导。CIA模型可用于阐明关节炎发展的相关机制，并经常用于研究新疗法的效果。最近在C57 BL/6小鼠中开发的CIA模型使研究人员能够使用基因敲除小鼠油这一背景进行关节炎研究。在本章中，描述了在两种小鼠品系中诱导关节炎的方案，包括在实验期间监测小鼠的临床关节炎和爪肿胀。此外，描述了用于爪脱钙和用于检测小鼠血清中的胶原特异性抗体的方案。
Collagen-induced arthritis (CIA) in mice is an animal model for rheumatoid arthritis (RA) and can be induced in DBA/1 and C57BL/6 mice using different protocols. The CIA model can be used to unravel mechanisms involved in the development of arthritis and is frequently used to study the effect of new therapeutics. The development of a CIA model in C57BL/6 mice recently enabled researchers to use knockout mice oil this background for arthritis research.In this chapter, the protocol for induction of arthritis in both mice strains is described, including the monitoring of clinical arthritis and paw swelling in the mice during the experiment. Furthermore, protocols for decalcification of paws and for the detection of collagen-specific antibodies in mice sera are described.