The bacterial RNA ligase RtcB accelerates the repair process of fragmented rRNA upon releasing the antibiotic stress
The bacterial RNA ligase RtcB accelerates the repair process of fragmented rRNA upon releasing the antibiotic stress
复制标题
细菌 RNA 连接酶 RtcB 在释放抗生素应激后加速片段化 rRNA 的修复过程
DOI：
10.1007/s11427-018-9405-y
复制
发表时间：
2020-02-01
影响因子：
9.1
通讯作者：
Yu, Jun
中科院分区：
文献类型：
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作者：
Manwar, Muhammad Ramzan;Shao, Changjun;Yu, Jun
RtcB是一种高度保守的RNA连接酶，存在于生命的所有三个领域，并被证明是古细菌和后生动物中必不可少的tRNA剪接成分。然而，在没有剪接的细菌中，RtcB的生物学功能仍有待阐明。我们首先进行了生物信息学分析，揭示了细菌中RtcB的高度保守结构和可能的保守功能。然而，它的同源物只存在于不同门中约0.5%的细菌物种中，具有频繁水平转移的显著信号，突出了它在细菌中的非必要作用。接下来，通过构建RtcB敲除菌株，我们发现抗生素胁迫的去除对野生型菌株RtcB表达有显著影响，并且RtcB （ARtcB菌株）的缺失延迟了恢复过程。我们的转录组学分析，包括rna的3 '端标记，突出了Δ RtcB菌株中未映射reads和裂解RNAs的显着增加，特别是在恢复期间。我们的观察结果表明，保守的RNA连接酶RtcB可以修复应激后受损的rnas，从而可能节省能量并加速其宿主的恢复。我们认为，不同细菌分类群对RtcB的获取提供了在逆境条件下的竞争优势。
RtcB, a highly conserved RNA ligase, is found in all three domains of life, and demonstrated to be an essential tRNA splicing component in archaea and metazoans. However, the biological functions of RtcB in bacteria, where there is no splicing, remains to be clarified. We first performed bioinformatics analysis which revealed highly conserved structures and presumably conserved functions of RtcB in bacteria. However, its orthologs only occur in ∼ 0.5% of bacterial species across diverse phyla with significant signals of frequent horizontal transfer, highlighting its non-essential role in bacteria. Next, by constructing anrtcB-knockout strain, we find that the removal of antibiotic stress induces a significant impact onrtcBexpression in wild-type strain, and furthermore the depletion of RtcB (ARtcB strain) delays the recovery process. Our transcriptomic analysis, comprising the 3′-end labeling of RNAs, highlights a significant increase in unmapped reads and cleaved rRNAs in the Δ RtcB strain, particularly during recovery. Our observations suggest that the conserved RNA ligase RtcB, repairs damaged rRNAs following stress, which potentially saves energy and accelerates recovery of its host. We propose that acquisition of RtcB by diverse bacterial taxa provides a competitive advantage under stressful conditions.