Maternal Polystyrene Microplastic Exposure during Gestation and Lactation Altered Metabolic Homeostasis in the Dams and Their F1 and F2 Offspring
Maternal Polystyrene Microplastic Exposure during Gestation and Lactation Altered Metabolic Homeostasis in the Dams and Their F1 and F2 Offspring
复制标题
妊娠期和哺乳期母体聚苯乙烯微塑料暴露改变了母鼠及其 F1 和 F2 后代的代谢稳态
DOI：
10.1021/acs.est.9b03191
复制
发表时间：
2019-09-17
影响因子：
11.4
通讯作者：
Jin, Yuanxiang
中科院分区：
文献类型：
--
作者：
Luo, Ting;Wang, Caiyun;Jin, Yuanxiang
微塑料是海洋环境的污染物，近年来已成为全球性的环境问题。许多研究表明，MP可以进入人类食物链，甚至在人类粪便中也检测到MP。因此，人们越来越关注MP对人类和动物健康的潜在风险。在此，我们研究了妊娠期和哺乳期母体聚苯乙烯MP暴露，并评价了对母体以及F1（PND 42和280）和F2（PND 42）代的潜在影响。转录组和16SrRNA测序结果表明，MPs导致母体MPs代谢紊乱，与肠道微生物群失调和肠道屏障功能障碍有关。同时，母体暴露于MPs也具有代际效应，甚至对F1和F2代的代谢产生长期影响。此外，在F1（PND 42）中，肠道微生物群的组成没有显著变化，而肝脏转录组和血清代谢物的变化显示了代谢紊乱的潜在风险。然后，在成年F1小鼠（PND 280）中观察到肝脏脂质蓄积的可能性，尤其是在雌性小鼠中。我们的研究结果表明，母体在妊娠期和哺乳期暴露于MP会增加代谢紊乱的风险，这些结果为MP的潜在长期危害提供了新的见解。
Microplastics (MPs) are considered as a pollutant of marine environments and have become a global environmental problem in recent years. A number of studies have demonstrated that MPs can enter the human food chain, and MPs have even been detected in human stools. Therefore, there is increasing concern about the potential risks of MPs to human and animal health. Here, we investigated maternal polystyrene MPs exposure during gestation and lactation and evaluated the potential effects on dams and the F1 (both PND 42 and 280) and F2 (PND 42) generations. The results of transcriptome and 16S rRNA sequencing indicated that MPs caused the metabolic disorder in maternal MPs associated with gut microbiota dysbiosis and gut barrier dysfunction. Simultaneously, maternal MPs exposure also had the intergenerational effects and even caused long-term metabolic consequences in the F1 and F2 generations. In addition, in F1 (PND 42), the composition of gut microbiota did not change significantly, while the hepatic transcriptome and serum metabolite changes showed the potential risk in metabolic disorder. Then, the potential of hepatic lipid accumulation was observed in adult F1 mice (PND 280), especially in the female mice. Our results demonstrated that maternal MPs exposure during gestation and lactation increases the risk of metabolic disorder, and these results provide new insight into the potential long-term hazards of MPs.