<scp>Micro‐CT</scp> analysis reveals the changes in bone mineral density in zebrafish craniofacial skeleton with age
<scp>Micro‐CT</scp> analysis reveals the changes in bone mineral density in zebrafish craniofacial skeleton with age
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<scp>Micro-CT</scp>分析揭示斑马鱼颅面骨骼骨密度随年龄的变化
DOI：
10.1111/joa.13780
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发表时间：
2022
影响因子：
2.4
通讯作者：
Tse William Ka Fai
中科院分区：
文献类型：
--
作者：
Liao Wei‐Neng;You May‐Su;Ulhaq Zulvikar Syambani;Li Jui‐Ping;Jiang Yun‐Jin;Chen Jen‐Kun;Tse William Ka Fai
骨骼在动物体内具有多种功能，例如支持身体活动。斑马鱼的骨骼由头面部骨骼和轴向骨骼组成。它的生理曲度与人类相同，由与人类相似数量的椎骨组成。作为人类疾病模型，斑马鱼的骨骼退化和畸形已被广泛研究。使用微型计算机断层扫描(Micro-CT)可以获得高分辨率的成像和不同的骨特性，如密度和体积。本研究旨在了解斑马鱼脊椎和头面部骨骼的结构和骨密度(BMD)随年龄的变化(受精后4、12和24个月[MPF])。结果表明，12 和24 mpf鱼的椎骨和特定的头面部骨骼(下颌弓、鳍骨和筛板)的骨密度均高于4 mpf鱼。此外，我们发现，面部骨骼中并不普遍存在与年龄相关的骨密度增加，而且这种差异与骨骼类型无关。总而言之，关于头面部骨骼的这些额外信息可能有助于理解斑马鱼整个生命周期中骨骼的发育。
Bone has multiple functions in animals, such as supporting the body for mobility. The zebrafish skeleton is composed of craniofacial and axial skeletons. It shares a physiological curvature and consists of a similar number of vertebrae as humans. Bone degeneration and malformations have been widely studied in zebrafish as human disease models. High‐resolution imaging and different bone properties such as density and volume can be obtained using micro‐computed tomography (micro‐CT). This study aimed to understand the possible changes in the structure and bone mineral density (BMD) of the vertebrae and craniofacial skeleton with age (4, 12 and 24 months post fertilisation [mpf]) in zebrafish. Our data showed that the BMD in the vertebrae and specific craniofacial skeleton (mandibular arch, ceratohyal and ethmoid plate) of 12 and 24 mpf fish were higher than that of the 4 mpf fish. In addition, we found the age‐dependent increase in BMD was not ubiquitously observed in facial bones, and such differences were not correlated with bone type. In summary, such additional information on the craniofacial skeleton could help in understanding bone development throughout the lifespan of zebrafish.