Phagocytosis of Escherichia coli biofilm cells with different aspect ratios: a role of substratum material stiffness.
Phagocytosis of Escherichia coli biofilm cells with different aspect ratios: a role of substratum material stiffness.
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不同纵横比的大肠杆菌生物膜细胞的吞噬作用：基质材料硬度的作用。
DOI：
10.1007/s00253-017-8394-2
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发表时间：
2017
影响因子：
5
通讯作者：
Ren,Dacheng
中科院分区：
文献类型：
--
作者：
Zhao,Yanrui;Song,Fangchao;Wang,Hao;Zhou,Junlin;Ren,Dacheng
细菌生物膜由于对抗生素的高度耐受性而在慢性感染中起重要作用。因此，重要的是根除附着在不同材料的植入医疗器械上的细菌细胞。吞噬作用是机体天然免疫系统清除入侵病原体的关键过程。以往的研究表明，吞噬效率是由聚合物珠的纵横比的影响。最近，我们报道了聚二甲基硅氧烷（PDMS）的刚性对大肠杆菌生物膜形成的影响，刚性（5：1）PDMS上的生物膜细胞比柔性（40：1）PDMS上的生物膜细胞短46.2%。基于这些发现，我们假设E.附着在刚性PDMS上的大肠杆菌细胞可以通过吞噬作用更有效地去除。在本研究中，使用活力测定、流式细胞术和细胞追踪来检验这一假设。结果表明，短体E.从硬PDMS表面脱离的大肠杆菌细胞比从软PDMS表面脱离的长细胞更容易被吞噬。此外，发现巨噬细胞在坚硬的PDMS表面上更具运动性，并且更有效地吞噬E。大肠杆菌细胞附着在这些表面上。这些结果可能有助于设计更好的生物材料，以减少污垢和相关的感染。
Bacterial biofilms play an important role in chronic infections due to high-level tolerance to antibiotics. Thus, it is important to eradicate bacterial cells that are attached to implanted medical devices of different materials. Phagocytosis is a key process of the innate immunity to eliminate invading pathogens. Previous research demonstrated that the efficiency of phagocytosis is affected by the aspect ratio of polymer beads. Recently, we reported that the stiffness of polydimethylsiloxane (PDMS) influencesEscherichia colibiofilm formation and the biofilm cells on stiff (5:1) PDMS are 46.2% shorter than those on soft (40:1) PDMS. Based on these findings, we hypothesized thatE. colicells attached on stiff PDMS can be more effectively removed via phagocytosis. This hypothesis was tested in the present study using viability assays, flow cytometry, and cell tracking. The results revealed that shorterE. colicells detached from stiff PDMS were easier to be phagocytized than the longer cells from soft PDMS surfaces. Furthermore, macrophage cells were found to be more motile on stiff PDMS surfaces and more effective at phagocytosis ofE. colicells attached on these surfaces. These results may help the design of better biomaterials to reduce fouling and associated infections.