Branchless encodes a Drosophila FGF homolog that controls tracheal cell migration and the pattern of branching
Branchless encodes a Drosophila FGF homolog that controls tracheal cell migration and the pattern of branching
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DOI：
10.1016/s0092-8674(00)81803-6
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发表时间：
1996-12-13
期刊：
影响因子：
64.5
通讯作者：
Krasnow, MA
中科院分区：
文献类型：
--
作者：
Sutherland, D;Samakovlis, C;Krasnow, MA
肺和昆虫气管系统等复杂器官结构模式的分子基础尚不清楚。在这里，我们描述了果蝇无分支基因（bnl），并证明它是气管分支模式的关键决定因素。 bnl 是气管分支所必需的，并且在新分支形成和生长的每个位置处的正在发育的气管系统周围的细胞簇中动态表达。 bnl 的局部错误表达可以引导分支形成并生长到新位置。普遍的错误表达激活了气管基因表达和分支的后期程序，从而产生了巨大的分支网络。 bnl 编码哺乳动物成纤维细胞生长因子 (FGF) 的同源物，并且似乎充当呼吸受体酪氨酸激酶的配体，呼吸受体酪氨酸激酶是在发育中的气管细胞上表达的 FGF 受体同源物。结果表明，该 FGF 途径通过在初级分支形成过程中引导气管细胞迁移，然后在生长的初级分支末端激活更精细分支的后续程序来指定气管分支模式。
The molecular basis for patterning of complex organ structures like the lung and insect tracheal system is unknown. Here, we describe the Drosophila gene branchless (bnl) and demonstrate that it is a key determinant of the tracheal branching pattern. bnl is required for tracheal branching and is expressed dynamically in clusters of cells surrounding the developing tracheal system at each position where a new branch will form and grow out. Localized misexpression of bnl can direct branch formation and outgrowth to new positions. Generalized misexpression activates later programs of tracheal gene expression and branching, resulting in massive networks of branches. bnl encodes a homolog of mammalian fibroblast growth factors (FGFs) and appears to function as a ligand for the breathless receptor tyrosine kinase, an FGF receptor homolog expressed on developing tracheal cells. The results suggest that this FGF pathway specifies the tracheal branching pattern by guiding tracheal cell migration during primary branch formation and then activating later programs of finer branching at the ends of growing primary branches.