Decreased mitochondrial DNA content in posttreatment salivary rinses from head and neck cancer patients
Decreased mitochondrial DNA content in posttreatment salivary rinses from head and neck cancer patients
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DOI：
10.1158/1078-0432.ccr-05-1471
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发表时间：
2006-03-01
影响因子：
11.5
通讯作者：
Califano, JA
中科院分区：
文献类型：
--
作者：
Jiang, WW;Rosenbaum, E;Califano, JA
目的与实验设计：在人类组织和肿瘤中，已发现线粒体DNA（mtDNA）序列和含量的改变与吸烟暴露有关。我们对细胞色素c氧化酶（Cox）I和Cox II基因进行定量PCR分析，以测量76例因原发性头颈部鳞状细胞癌接受手术切除的患者在治疗前和治疗后的唾液冲洗液中mtDNA含量的变化。我们还研究了mtDNA含量变化与术后放疗、吸烟暴露、饮酒及其他临床特征之间的关系。
结果：总体而言，治疗后唾液中的mtDNA含量显著降低。Cox I的平均变化为 -0.21[95%置信区间（95%CI），-0.44至0.01，P = 0.06]，Cox II的平均变化为 -0.31（95%CI，-0.55至 -0.08，P = 0.01）。放疗组患者与未放疗组相比，含量显著降低（Cox I的P = 0.03；Cox II的P = 0.05）。此外，在从不吸烟的患者中发现Cox I（-0.71；95%CI，-1.17至 -0.25，P = 0.005）和Cox II（-0.65；95%CI，-1.17至 -0.13，P = 0.02）显著降低，但在既往吸烟者或当前吸烟者中未发现。
结论：我们的数据表明，在从不吸烟者以及原发性手术切除后接受放疗的患者中，唾液mtDNA含量降低。
Purpose and Experimental Design: Alterations in mitochondrial DNA (mtDNA) sequence and content have been described in human tissues and tumors in association with smoking exposure. We did quantitative PCR analysis of cytochrome c oxidase (Cox) I and Cox II genes to measure changes in mtDNA content in pretreatment and posttreatment salivary rinses obtained from 76 patients undergoing surgical resection for primary head and neck squamous cell carcinoma. We also examined the relationship between changes in mtDNA content and postoperative radiation therapy, smoking exposure, alcohol intake, and other clinical characteristics.Results: Overall, mtDNA content in posttreatment saliva was significantly decreased. The mean change for Cox I was -0.21 [95% confidence interval (95% CI), -0.44 to 0.01, P = 0.06] and for Cox II was -0.31 (95% Cl, -0.55 to -0.08, P = 0.01). Patients in the radiation therapy group exhibited a significant decrease compared with the nonradiated group (P = 0.03 for Cox I; P = 0.05 for Cox II). In addition, significant decreases in Cox /I(-0.71; 95% Cl, -1.17 to -0.25, P = 0.005) and Cox II (-0.65; 95% Cl, -1.17 to -0.13, P = 0.02) were found in never-smoking patients but not in former or current smokers.Conclusion: Our data suggest that salivary mtDNA content is decreased in never smokers and in response to radiation therapy after primary surgical resection.