RNP-Based Control Systems for Genetic Circuits in Synthetic Biology Beyond CRISPR
RNP-Based Control Systems for Genetic Circuits in Synthetic Biology Beyond CRISPR
复制标题
CRISPR 之外的合成生物学中基于 RNP 的遗传电路控制系统
DOI：
10.1007/978-1-0716-2421-0_1
复制
发表时间：
2022
影响因子：
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通讯作者：
Trevor R Simmons 1, Andrew D
中科院分区：
文献类型：
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作者：
Trevor R Simmons 1, Andrew D
Ribonucleoproteins (RNPs) are RNA-protein complexes utilized natively in both prokaryotes and eukaryotes to regulate essential processes within the cell. Over the past few years, many of these native systems have been adapted to provide control over custom genetic targets. Engineered RNP-based control systems allow for fine-tune regulation of desired targets, by providing customizable nucleotide-nucleotide interactions. However, as there have been several engineered RNP systems developed recently, identifying an optimal system for various bioprocesses is challenging. Here, we review the most successful engineered RNP systems and their applications to survey the current state of the field. Additionally, we provide selection criteria to provide users a streamlined method for identifying an RNP control system most useful to their own work. Lastly, we discuss future applications of RNP control systems and how they can be utilized to address the current grand challenges of the synthetic biology community.
