The microRNA spectrum in 12 body fluids.
The microRNA spectrum in 12 body fluids.
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DOI：
10.1373/clinchem.2010.147405
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发表时间：
2010-11
影响因子：
9.3
通讯作者：
Wang K
中科院分区：
文献类型：
--
作者：
Weber JA;Baxter DH;Zhang S;Huang DY;Huang KH;Lee MJ;Galas DJ;Wang K
MicroRNA (miRNA) 是小型非编码 RNA，通过与细胞信使 RNA 相互作用，在调节各种生物过程中发挥重要作用。血清、血浆、唾液和尿液中的细胞外 miRNA 最近已被证明与包括癌症在内的各种病理状况有关。为了评估 miRNA 的分布并证明 miRNA 作为生物标志物的潜在用途，我们检查了 12 种人体体液和不同妊娠阶段女性或患有不同尿路上皮癌患者的尿液样本中 miRNA 的存在情况。我们使用定量 PCR 对这些液体中的 miRNA 分布进行了全球调查。 miRNA 存在于所有测试的液体中，并且在不同的液体类型中显示出不同的组成。这些液体中的一些高丰度 miRNA 在多种液体类型中很常见，并且一些 miRNA 在特定液体中富集。我们还观察到从具有不同病理生理状况的个体获得的尿液样本中存在不同的 miRNA 模式。 MicroRNA 在所有测试的体液类型中普遍存在。液体类型特异性 miRNA 可能具有与周围组织相关的功能作用。此外，在患有不同尿路上皮疾病的患者的尿液样本中观察到的 miRNA 光谱的变化表明，使用体液中特定 miRNA 的浓度作为检测和监测各种病理生理学状况的生物标志物的潜力。
MicroRNAs (miRNAs) are small, noncoding RNAs that play an important role in regulating various biological processes through their interaction with cellular messenger RNAs. Extracellular miRNAs in serum, plasma, saliva, and urine have recently been shown to be associated with various pathological conditions including cancer. With the goal of assessing the distribution of miRNAs and demonstrating the potential use of miRNAs as biomarkers, we examined the presence of miRNAs in 12 human body fluids and urine samples from women in different stages of pregnancy or patients with different urothelial cancers. Using quantitative PCR, we conducted a global survey of the miRNA distribution in these fluids. miRNAs were present in all fluids tested and showed distinct compositions in different fluid types. Several of the highly abundant miRNAs in these fluids were common among multiple fluid types, and some of the miRNAs were enriched in specific fluids. We also observed distinct miRNA patterns in the urine samples obtained from individuals with different physiopathological conditions. MicroRNAs are ubiquitous in all the body fluid types tested. Fluid type–specific miRNAs may have functional roles associated with the surrounding tissues. In addition, the changes in miRNA spectra observed in the urine samples from patients with different urothelial conditions demonstrates the potential for using concentrations of specific miRNAs in body fluids as biomarkers for detecting and monitoring various physiopathological conditions.