Abundance and persistence of antibiotic resistance genes in livestock farms: A comprehensive investigation in eastern China
Abundance and persistence of antibiotic resistance genes in livestock farms: A comprehensive investigation in eastern China
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DOI：
10.1016/j.envint.2013.08.023
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发表时间：
2013-11-01
影响因子：
11.8
通讯作者：
Yan, Shuhai
中科院分区：
文献类型：
--
作者：
Cheng, Weixiao;Chen, Hong;Yan, Shuhai
环境中抗生素耐药性基因的增加可能会对公众健康构成威胁。本研究旨在调查杭州市8个养殖场中四环素(Tet)和磺胺(Sul)抗性基因的丰度和多样性。采用实时定量聚合酶链式反应技术，对10个tet基因(TetA、TetB、TetC、TETG、TETL、TETM、TETO、TETQ、TETW和TETX)、2个SuL基因(Gull和SulII)和1个与耐药基因相关的基因元件[1类整合子(IntI1)]进行了定量检测。不同规模的猪场、鸡场和鸭场的tet和sul基因丰度差异不显著(P>；0.05)。粪便和废水样品中核糖体保护蛋白基因的平均丰度高于大多数外排泵基因(TetA、TETB、TETC和TETL)和酶修饰基因(TETX)(P<；0.05)，但外排泵基因TETG含量较高，与TETM差异不显著。即使在处理后，大多数ARGs在废水泻湖中的相对丰度也高于在粪便中的相对丰度。尽管三个核糖体保护蛋白基因(ETQ、TETW和TETO)具有较高的相对丰度，但在养猪场的整个废水处理过程中，数量减少(P<；0.05)。经污水处理后，它们的相对丰度增加，并且这三个基因的去除与intI1基因的表达呈显著的正相关(分别为：ETG：R-2=0.60，P<；0.05；Suli：R-2=0.72，P<；0.05；sulII：R-2=0.62，P<；0.05)，提示intI1可能参与了它们的增殖。猪场和鸭场粪便样本中的TetM和sulII基因差异极显著(P<；0.001)。系统发育分析表明，TETM在鸭场的多样性高于在猪场的多样性。此外，猪场和鸭场的SulII序列都是保守的。这是第一次详细说明动物粪便和农业废水处理系统中特定ARGs相对丰富的综合研究，可能为管理农业活动产生的抗生素耐药性提供知识。(C)2013爱思唯尔有限公司。保留所有权利。
Increases of antibiotic resistance genes in the environment may pose a threat to public health. The purpose of this study was to investigate the abundance and diversity of tetracycline (tet) and sulfonamide (sul) resistance genes in eight livestock farms in Hangzhou, eastern China. Ten tet genes (tetA, tetB, tetC, tetG,tetL, tetM, tetO, tetQ tetW, and tetX), two sul genes (gull and sulII), and one genetic element associated with mobile antibiotic resistance genes [class 1 integron (intI1)] were quantified by real-time polymerase chain reaction. No significant difference was found in the abundance of the tet and sul genes in various scales of pig, chicken, and duck farms (P > 0.05). The average abundance of ribosomal protection protein genes (tetQ tetM, tetW, and tetO) in the manure and wastewater samples was higher than most of the efflux pump genes (tetA, tetB, tetC, and tetL) and enzymatic modification gene (tetX) (P < 0.05), except for efflux pump gene tetG, which was abundant and showed no difference from tetM. Most ARGs had higher relative abundance in the wastewater lagoon than in manures even after treatment. Although the three ribosomal protection protein genes (tetQ, tetW, and tetO) had higher relative abundance, numbers were reduced during the complete wastewater treatment process in pig farms (P < 0.05). The relative abundance of tetG, sulI, and sulII increased after the wastewater treatment and the removal of these three genes exhibited significant positive correlations with the intI1 gene (tetG: R-2 = 0.60, P < 0.05; sulI: R-2 = 0.72, P < 0.05; sulII: R-2 = 0.62, P < 0.05), suggesting that intI1 may be involved in their proliferation. As for tetM and sulII genes, a highly significant difference was found in manure samples between pig farms and duck farms (P < 0.001). Phylogenetic analysis showed that tetM was more diverse in duck farms than in pig farms. Additionally, sulII sequence was conserved both in pig and duck farms. This is the first comprehensive study to detail the relative abundance of specific ARGs in animal manures and agricultural wastewater treatment systems, potentially providing knowledge for managing antibiotic resistance emanating from agricultural activities. (C) 2013 Elsevier Ltd. All rights reserved.