Towards sequential bioethanol and L-lactic acid co-generation: Improving xylose conversion to L-lactic acid in presence of lignocellulosic ethanol with an evolved Bacillus coagulans
Towards sequential bioethanol and L-lactic acid co-generation: Improving xylose conversion to L-lactic acid in presence of lignocellulosic ethanol with an evolved Bacillus coagulans
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DOI：
10.1016/j.renene.2020.02.066
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发表时间：
2020-06-01
期刊：
影响因子：
8.7
通讯作者：
Tomas-Pejo，Elia
中科院分区：
文献类型：
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作者：
Cubas-Cano，Enrique;Pablo Lopez-Gomez，Jose;Tomas-Pejo，Elia
考虑到木质纤维素的高成分多样性，它可以生产各种生物燃料和生物基产品。酵母从纤维素葡萄糖中生产乙醇是众所周知的，而半纤维素木糖的利用仍然具有挑战性。本研究提出利用木糖进行乳酸发酵。在此背景下，研究了酿酒酵母的序贯培养和利用凝固芽孢杆菌的c5。高乙醇产量，约0.44 g g−1，从纤维素园艺水解物获得。高浓度乙醇对进化db没有影响。混凝物a20 - exa通过适应进化得到乙醇。结果表明，与亲本b相比，乳酸产率提高2.6倍。在5% （v v−1）乙醇存在下凝固菌株。这些结果证明了b。凝固剂a20 - exa与s合用。在生物炼制方法中，用于木质纤维素生物质连续热电联产乙醇和乳酸的酿酒。
A wide range of biofuels and bio-based products can be produced from lignocellulose considering its high compositional diversity. Ethanol production by yeasts from cellulosic glucose is well-known, while hemicellulosic xylose utilization is still challenging. This work proposes the use the xylose forl-lactic acid fermentation. In this context, a sequential cultivation ofSaccharomycescerevisiaeand the C5-utilizingBacillus coagulansis studied. High ethanol yields, around 0.44 g g−1, were obtained from a cellulosic-gardening hydrolysate. The high ethanol concentrations did not affect the evolvedB. coagulansA20-EXA obtained by adaptive evolution to ethanol. As a result, 2.6-fold increase in lactic acid yield was achieved when compared with parentalB. coagulansstrain in presence of 5% (v v−1) ethanol. These results demonstrated the suitability ofB. coagulansA20-EXA to be used together withS. cerevisiaefor the sequential co-generation of ethanol and lactic acid from lignocellulosic biomass in a biorefinery approach.