Synthesis of luminescent squaramide monoesters: cytotoxicity and cell imaging studies in HeLa cells
Synthesis of luminescent squaramide monoesters: cytotoxicity and cell imaging studies in HeLa cells
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DOI：
10.1039/c5ra24521d
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发表时间：
2016-01-01
期刊：
影响因子：
3.9
通讯作者：
Concepcion Gimeno, M.
中科院分区：
文献类型：
--
作者：
Fernandez-Moreira, Vanesa;Alegre-Requena, Juan V.;Concepcion Gimeno, M.
本文首次合成了具有不同荧光基团的新型发光方酰胺单酯，并在细胞成像方面进行了探索。在HeLa宫颈癌细胞中进行的细胞毒性研究显示，这些新结构中的一些具有高活性，突出了荧光片段在这些有前途的抗癌药物效率中的重要性。此外，荧光细胞显微镜揭示了不同的生物分布行为取决于荧光部分，以及核定位的可能性手性非平面方酰胺单酯。
Novel luminescent squaramide monoesters functionalised with different fluorophore groups have been synthesised and explored in cell imaging for the first time. Cytotoxicity studies performed in HeLa cervical cancer cells revealed high activity for some of these novel structures, highlighting the importance of the fluorescent fragment in the efficiency of these promising anticancer agents. In addition, fluorescence cell microscopy disclosed the different biodistribution behaviour depending on the fluorescent moiety, and the possibility of nuclear localisation of chiral non planar squaramide monoesters.