The protective effect of epigallocatechin 3-gallate on mouse pancreatic islets via the Nrf2 pathway
The protective effect of epigallocatechin 3-gallate on mouse pancreatic islets via the Nrf2 pathway
复制标题
DOI：
10.1007/s00595-019-1761-0
复制
发表时间：
2019-02
期刊：
影响因子：
2.5
通讯作者：
Y. Wada;Atsushi Takata;T. Ikemoto;Y. Morine;S. Imura;S. Iwahashi;Yu Saito;M. Shimada
中科院分区：
文献类型：
--
作者：
Y. Wada;Atsushi Takata;T. Ikemoto;Y. Morine;S. Imura;S. Iwahashi;Yu Saito;M. Shimada
表没食子儿茶素3-没食子酸酯（EGCG）是一种绿茶多酚，已被证明具有体外和体内抗氧化和抗炎作用。本研究的目的是探讨EGCG作为胰岛移植的预处理对离体胰岛的影响及其机制。方法将胰岛分为两组：胰岛培养基组（对照组）和胰岛培养基加EGCG（100µM）组。我们研究了胰岛活力、Nrf2表达、活性氧（ROS）产生和血红素加氧酶-1 (HO-1) mRNA。将100µM EGCG和对照组培养12 h后的500个胰岛当量移植到链脲佐菌素诱导的糖尿病ICR小鼠肾囊下。结果EGCG组细胞活力和胰岛素分泌能力保持不变，Nrf2核易位增加（p< 0.01）。EGCG组HO-1 mRNA表达量高于对照组（p< 0.05）， ROS表达量低于对照组（p< 0.01）。体内功能评估显示，EGCG组移植后血糖水平下降（p< 0.01）。结论egcg通过Nrf2途径抑制ROS的产生，保护胰岛细胞的活力和功能。
PurposeEpigallocatechin 3-gallate (EGCG), a green tea polyphenol, has been shown to have anti-oxidant and anti-inflammatory effects in vitro and in vivo. The aim of this study was to investigate the effects and mechanism of EGCG on isolated pancreatic islets as pre-conditioning for pancreatic islet transplantation.MethodsThe pancreatic islets were divided into two groups: an islet culture medium group (control) and an islet culture medium with EGCG (100 µM) group. We investigated the islet viability, Nrf2 expression, reactive oxygen species (ROS) production, and heme oxygenase-1 (HO-1) mRNA. Five hundred islet equivalents after 12 h of culture for the EGCG 100 µM and control group were transplanted under the kidney capsule of streptozotocin-induced diabetic ICR mice.ResultsThe cell viability and insulin secretion ability in the EGCG group were preserved, and the nuclear translocation of Nrf2 was increased in the EGCG group (p< 0.01). While the HO-1 mRNA levels were also higher in the EGCG group than in the control group (p< 0.05), the ROS production was lower (p< 0.01). An in vivo functional assessment showed that the blood glucose level had decreased in the EGCG group after transplantation (p< 0.01).ConclusionEGCG protects the viability and function of islets by suppressing ROS production via the Nrf2 pathway.