Acetylcholine-induced receptor-controlled ion flux investigated by flow quench techniques.

Acetylcholine-induced receptor-controlled ion flux investigated by flow quench techniques.
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通过流动猝灭技术研究乙酰胆碱诱导的受体控制的离子通量。

DOI:
10.1016/0006-291x(80)91573-9
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发表时间:
1980
影响因子:
3.1
通讯作者:
Hess,GP
Hess,GP
中科院分区:
生物学4区
文献类型:
--
作者:
Cash,DJ;Aoshima,H;Hess,GP

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Using a quench flow technique with membrane vesicles, the acetylcholine receptor-controlled transmembrane ion flux and the inactivation of the receptor with acetylcholine were measured in the msec time region. The ion flux was followed by influx of radioactive tracer ion and the inactivation was followed by an ion flux assay of receptor pre-incubated with ligand. The measurements covered a concentration range to complete saturation of the active state of the receptor with ligand, and were consistent with a minimal model previously proposed on the basis of experiments with carbamylcholine. The ion translocation rate at saturation with acetylcholine is about twice that at saturation with carbamylcholine and this reflects a more favored channel opening equilibrium for acetylcholine.
DOI: 10.1113/jphysiol.1953.sp004849
发表时间: 1953-01-01
影响因子: 5.5
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期刊: Biochemical and Biophysical Research Communications - BBRC
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发表时间: 1957
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DOI: 10.1016/0006-291x(76)90950-5
发表时间: 1976
影响因子: 3.1
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通讯作者: G. Struve