Biofilm formation by Aeromonas strains under food-related environmental stress conditions
Biofilm formation by Aeromonas strains under food-related environmental stress conditions
复制标题
食品相关环境应激条件下气单胞菌菌株生物膜的形成
DOI：
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
R. Shashidhar
中科院分区：
文献类型：
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作者：
V. Nagar;Lipika Pansare Godambe;J. Bandekar;R. Shashidhar
气单胞菌是重要的人类和鱼类病原体，可以在食品和食品接触表面形成生物膜，可能成为食品加工设施中交叉污染的来源。使用基于聚苯乙烯微量滴定板的结晶紫测定法进行了一项调查，以评估各种食品相关条件（培养基成分、温度、pH、盐和食品防腐剂）对属于 6 个物种的 10 种不同气单胞菌菌株生物膜形成的影响。这些菌株的生物膜形成能力差异很大，其中大多数在 TSB 和 M9 基本培养基中产生微弱的生物膜。在各种条件下，大多数菌株在低温（10℃）和酸性条件（pH 5）下形成最高的生物膜。大多数菌株的生物膜形成随着 NaCl 浓度的增加而减少。结果表明，食品环境中遇到的各种非生物胁迫都会影响气单胞菌菌株生物膜的产生，并且主要是菌株特异性现象。
实际应用
本研究报告了气单胞菌属物种在不同的食品相关环境胁迫条件下形成的生物膜。它将帮助食品加工商和监管机构优化禁止措施，以防止生物膜形成并减少与生物膜形成气单胞菌菌株相关的健康风险。
Aeromonas, important human and fish pathogens, can form biofilm on food and food-contact surfaces that may act as a source of cross-contamination in the food-processing facilities. An investigation was undertaken to evaluate the impact of various food-related conditions (media composition, temperature, pH, salt, and food preservatives) on the biofilm formation by 10 different Aeromonas strains, belonging to six species, using polystyrene microtiter plate-based crystal-violet assay. These strains were vastly diverse in their biofilm forming abilities with the majority of them producing weak biofilm in TSB and M9 minimal medium. Among various conditions, majority of the strains formed the highest biofilm at low temperature (10°C) and acidic condition (pH 5). Biofilm formation for most of the strains reduced with an increase in NaCl concentration. The results indicate that various abiotic stresses encountered in food environment affect the production of biofilm in Aeromonas strains and is mainly a strain-specific phenomenon.
Practical applications
The present study reports the biofilm formation by Aeromonas species under different food-related environmental stress conditions. It will help food processors and regulators to optimize prohibitive measures to prevent biofilm formation and reduce the health risks related to biofilm-forming Aeromonas strains.
影响因子：
2.8
作者：
Khajanchi, Bijay K.;Sha, Jian;Chopra, Ashok K.
通讯作者：
Chopra, Ashok K.