SITE-SPECIFIC DNA ENDONUCLEASE AND RNA MATURASE ACTIVITIES OF 2 HOMOLOGOUS INTRON-ENCODED PROTEINS FROM YEAST MITOCHONDRIA
SITE-SPECIFIC DNA ENDONUCLEASE AND RNA MATURASE ACTIVITIES OF 2 HOMOLOGOUS INTRON-ENCODED PROTEINS FROM YEAST MITOCHONDRIA
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DOI：
10.1016/0092-8674(89)90246-8
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发表时间：
1989-02-10
期刊：
影响因子：
64.5
通讯作者：
JACQ, C
中科院分区：
文献类型：
--
作者：
DELAHODDE, A;GOGUEL, V;JACQ, C
酿酒酵母线粒体基因组777-3A的两个内含子，cob基因中的bl4和cox1基因中的al4，含有可翻译成两种同源蛋白的开放阅读框。我们将这些ORF的UGA、AUA和CUN密码子改为通用的遗传密码，以便通过将核编码蛋白重定向到线粒体来研究它们在大肠杆菌和酵母中的翻译产物的功能。P27bl4蛋白已被证明是剪接内含子bl4和al4所必需的。同源的p28al4蛋白对大肠杆菌有很强的毒性。它可以在代表两个融合的侧翼外显子交界处的序列上特异性地切割双链DNA。我们提出的证据表明，该系统是研究线粒体内含子编码蛋白在RNA(RNA剪接-BL4成熟酶)和DNA(内含子转座-AL4转座酶)水平上遗传信息重排中作用的良好模型。
Two introns of the mitochondrial genome 777-3A of Saccharomyces cerevisiae, bl4 in cob and al4 in coxl genes, contain ORFs that can be translated into two homologous proteins. We changed the UGA, AUA, and CUN codons of these ORFs to the universal genetic code, in order to study the functions of their translated products in Escherichia coli and in yeast, by retargeting the nuclear encoded protein into mitochondria. The p27bl4 protein has been shown to be required for the splicing of both introns bl4 and al4. The homologous p28al4 protein is highly toxic to E. coli. It can specifically cleave double-stranded DNA at a sequence representing the junction of the two fused flanking exons. We present evidence that this system is a good model for studying the role of mitochondrial intron-encoded proteins in the rearrangement of genetic information at both the RNA (RNA splicing-bl4 maturase) and DNA levels (intron transposition-al4 transposase).