A quantum dot conjugated sugar ball and its cellular uptake on the size effects of endocytosis in the subviral region
A quantum dot conjugated sugar ball and its cellular uptake on the size effects of endocytosis in the subviral region
复制标题
DOI：
10.1021/ja048792a
复制
发表时间：
2004-06-02
影响因子：
15
通讯作者：
Aoyama, Y
中科院分区：
文献类型：
--
作者：
Osaki, F;Kanamori, T;Aoyama, Y
用三辛基氧化膦（TOPOQD）包被的亲脂性CdSe量子点（QD）可以在与大环糖簇两亲物1相互作用时从氯仿中萃取到水中。因此，在水中溶解的15nm大小的QD缀合的和高荧光的糖球（TOPOQD）容易通过胞吞作用侵入Hela细胞。TOPOQD 1（15 nm）的内吞活性，根据1的胶束同质聚集体（5 nm）和病毒样1 −DNA缀合物（50 nm）的内吞活性作为参考，揭示了在亚病毒大小区域中的显著大小效应（50 nm 15 nm 5）。最佳尺寸为50 nm，表明在小的主客体系统中控制分子识别的尺寸互补性也在作为大生物分子宿主的内吞囊泡（≤100 nm）对纳米客体颗粒的包封中起关键作用。因此，这项工作表明，在设计分子（基因、药物、探针等）时，对病毒大小的控制至关重要。送货机器
The lipophilic CdSe quantum dot (QD) coated with trioctylphosphine oxide (TOPOQD) can be extracted from chloroform into water upon interaction with macrocyclic glycocluster amphiphile1. The QD-conjugated and highly fluorescent sugar ball of a size of 15 nm (TOPOQD) thus solubilized in water readily invades Hela cells via endocytosis. The endocytic activity ofTOPOQD1(15 nm), in light of those of the micellar homoaggregate of1(5 nm) and the virus-like1−DNA conjugate (50 nm) as references, reveals a dramatic size effect (50 ≫ 15 ≫ 5) in the subviral size region. The optimal size at ∼50 nm indicates that size complementarity which governs molecular recognition in small host−guest systems also plays key roles in the encapsulation of nanometric guest particles by the endocytic vesicles (≤100 nm) as a macrobiomolecular host. The work thus suggests an utmost importance of size control at the viral size when designing molecular (gene, drug, probe, etc.) delivery machines.