Novel fluorogenic substrates for imaging 6-lactamase gene expression
Novel fluorogenic substrates for imaging 6-lactamase gene expression
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DOI：
10.1021/ja036126o
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发表时间：
2003-09-17
影响因子：
15
通讯作者：
Rao, JH
中科院分区：
文献类型：
--
作者：
Gao, WZ;Xing, BG;Rao, JH
一种新的小的非荧光底物在β-内酰胺酶水解后变得明亮的荧光，荧光强度增加了153倍。可以很容易地检测到细胞裂解物中低于500fM的β-内酰胺酶，并且可以用红色荧光衍生物来成像活细胞中β-内酰胺酶的表达。这些新的荧光底物将在临床诊断中得到应用，并促进β-内酰胺酶作为生物传感器的应用。
A new class of small nonfluorescent fluorogenic substrates becomes brightly fluorescent after β-lactamase hydrolysis with up to 153-fold enhancement in the fluorescence intensity. Less than 500 fM of β-lactamase in cell lysates can be readily detected, and β-lactamase expression in living cells can be imaged with a red fluorescence derivative. These new fluorogenic substrates should find uses in clinical diagnostics and facilitate the applications of β-lactamase as a biosensor.