Insight into Emiliania huxleyi coccospheres by focused ion beam sectioning
Insight into Emiliania huxleyi coccospheres by focused ion beam sectioning
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DOI：
10.5194/bg-12-825-2015
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发表时间：
2015-01-01
期刊：
影响因子：
4.9
通讯作者：
Scheu, C.
中科院分区：
文献类型：
--
作者：
Hoffmann, R.;Kirchlechner, C.;Scheu, C.
在指数生长期对培养的 Emiliania huxleyi 克隆的球球进行取样，并使用聚焦离子束显微镜进行切片。每个球球平均有 69 个切片和相应的二次电子显微照片，提供了球球结构的详细信息。球石球平均有 2-3 层，每个细胞有 20 个球石，而在传统的扫描电子显微照片中只能看到其中 15 个。球石球外径与球石层数呈正相关。相比之下，内球球直径（约 4.36 μm）以及细胞直径是准恒定的。颗石石在整个颗石球中的分布并不均匀，导致颗石球的一个部分往往比另一部分显示出更多的颗石层。架构数据允许计算 PIC / POC 比率、单个细胞的密度和下沉速度。这些参数的相关性对正在进行的关于球石功能的争论具有重要意义。
Coccospheres of a cultured Emiliania huxleyi clone were sampled in the exponential growth phase and sectioned using a focused ion beam microscope. An average of 69 sections and the corresponding secondary electron micrographs per coccosphere provided detailed information on coccosphere architecture. The coccospheres feature 2-3 layers on average and 20 coccoliths per cell, of which only 15 can be seen in conventional scanning electron micrographs. The outer coccosphere diameter was positively correlated with the number of coccolith layers. By contrast, the inner coccosphere diameter (around 4.36 mu m), and hence the cell diameter, was quasi-constant. Coccoliths were not evenly distributed across the coccosphere, resulting more often than not in one part of the coccosphere displaying more coccolith layers than the other. The architectural data allowed for the calculation of the PIC / POC ratio, the density and the sinking velocity of individual cells. The correlation of these parameters has implications for the ongoing debate on the function of coccoliths.