Three-dimensional printed strontium-containing mesoporous bioactive glass scaffolds for repairing rat critical-sized calvarial defects
Three-dimensional printed strontium-containing mesoporous bioactive glass scaffolds for repairing rat critical-sized calvarial defects
复制标题
三维打印含锶介孔生物活性玻璃支架，用于修复大鼠临界尺寸的颅骨缺损。
DOI：
10.1016/j.actbio.2014.10.015
复制
发表时间：
2015-01-15
影响因子：
9.7
通讯作者：
Zhang, Changqing
中科院分区：
文献类型：
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作者：
Zhao, Shichang;Zhang, Jianhua;Zhang, Changqing
开发新一代具有高成骨能力的生物材料，使其与宿主骨快速骨结合，正在被广泛研究。在本研究中，我们通过三维打印技术制备了三维含锶介孔生物活性玻璃(sr-MBG)支架。SR-MBG支架大孔连通均匀(约400微米)，孔隙率高(约70%)，抗压强度高(8.67+/-1.74 Mpa)。以MBG支架为对照，通过成骨细胞样细胞MC3T3-E1的磷灰石形成能力、黏附、增殖、碱性磷酸酶活性和成骨基因表达等指标评价锶-MBG支架的生物学特性。此外，将锶-MBG支架用于修复临界大小的大鼠颅骨缺损。结果表明，锶-MBG支架具有良好的磷灰石形成能力，并能促进MC3T3-E1细胞的增殖和分化。重要的是，体内实验结果显示，锶-MBG支架具有良好的成骨能力，并在8周内刺激临界大鼠颅骨缺损区新生血管的形成。因此，具有良好孔结构和高成骨能力的三维打印锶-MBG支架在骨再生方面具有更多的潜在应用。(C)2014 Acta Materialia Inc.由Elsevier Ltd.出版。保留所有权利。
The development of a new generation of biomaterials with high osteogenic ability for fast osseointegration with host bone is being intensively investigated. In this study, we have fabricated three-dimensional (3-D) strontium-containing mesoporous bioactive glass (Sr-MBG) scaffolds by a 3-D printing technique. Sr-MBG scaffolds showed uniform interconnected macropores (similar to 400 mu m), high porosity (similar to 70%) and enhanced compressive strength (8.67 +/- 1.74 MPa). Using MBG scaffolds as a control, the biological properties of Sr-MBG scaffolds were evaluated by apatite-forming ability, adhesion, proliferation, alkaline phosphatase activity and osteogenic gene expression of osteoblast-like cells MC3T3-E1. Furthermore, Sr-MBG scaffolds were used to repair critical-sized rat calvarial defects. The results showed that Sr-MBG scaffolds possessed good apatite-forming ability and stimulated MC3T3-E1 cell proliferation and differentiation. Importantly, the in vivo results revealed that Sr-MBG scaffolds had good osteogenic capability and stimulated new blood vessel formation in critical-sized rat calvarial defects within 8 weeks. Therefore, 3-D printed Sr-MBG scaffolds with favorable pore structure and high osteogenic ability have more potential applications in bone regeneration. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.