Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1.
Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1.
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DOI：
10.1093/dnares/2.4.167
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发表时间：
1995-08-31
期刊：
影响因子：
--
通讯作者：
Nomura, N
中科院分区：
文献类型：
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作者：
Nagase, T;Seki, N;Nomura, N
在这一系列关于人类未知基因序列信息积累的项目中，我们新推导了40个人类细胞系KG-1全长cDNA克隆的序列，并预测了相应基因的编码序列，命名为KIAA 0121至0160。计算机检索公共数据库的结果表明，13个基因的序列与任何已报道的基因无关，而其余27个基因的序列与已知基因有一定的相似性。观察到的明显独特序列如下。三个基因中的每一个都含有一段三联重复序列：KIAA 0122和KIAA 0147中的GAG（Glu），以及KIAA 0150中的TCC（Ser）。KIAA 0139中一段10个氨基酸残基的序列重复了21次，KIAA 0125的非翻译区中有一段76-78个核苷酸的同源序列重复了6次。北方杂交分析表明，13个基因的表达在细胞或组织特异性的方式。尽管大量的表达序列标签（expressedsequencetags，ESTs）已被注册用于cDNA克隆的综合分析，但我们的序列数据表明它们的分布是非常不平衡的：例如，当没有ESTs命中7个基因时，85个ESTs落在单个基因中。
In this series of projects regarding the accumulation of sequence information of unidentified human genes, we newly deduced the sequences of 40 full-length cDNA clones of human cell line KG-1, and predicted the coding sequences of the corresponding genes, named KIAA0121 to 0160. The results of a computer search of public databases indicated that the sequences of 13 genes were unrelated to any reported genes, while the remaining 27 genes carried sequences which showed some similarities to known genes. Obvious unique sequences noted were as follows. A stretch of triplet repeats was contained in each of three genes: These were GAG(Glu) in KIAA0122 and KIAA0147, and TCC(Ser) in KIAA0150. A stretch of 10 amino acid-residues was repeated 21 times in KIAA0139, and a homologous sequence of 76-78 nucleotides was found repeated 6 times in the untranslated region of KIAA0125. Northern hybridization analysis demonstrated that 13 genes were expressed in a cell- or tissue-specific manner. Although a vast number of expressed sequence tags (ESTs) have been registered for comprehensive analysis of cDNA clones, our sequence data indicated that their distribution is very unbalanced: e.g. while no EST hit 7 genes, 85 ESTs fell in a single gene.