Evidence for an additional cattle DQB locus
Evidence for an additional cattle DQB locus
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额外牛 DQB 基因座的证据
作者：
A. Gelhaus;B. Förster;R. Horstmann
摘要通过对牛主要组织相容性复合体II类基因的遗传多态性研究，我们发现了一个类似于II类β链的基因序列（KUH 1）。 该基因在外周血白细胞中转录。序列比较、Southern杂交和系统发育分析表明：（1）KUH 1代表一个独特的DQB基因座，我们建议将其命名为BoLA-DQB 5;（2）DQB 5是一个古老的DQB基因座，它在DQB 1和DQB 2重复之前从一个共同的祖先基因分化出来;
Abstract Studying the genetic polymorphism of the major histocompatibility complex class II genes in cattle, we identified a sequence (KUH1) which resembles those encoding class II β chains. The gene was shown to be transcribed in peripheral blood leukocytes. Sequence comparisons, Southern blot, and phylogenetic analyses indicate that (1) KUH1 represents a distinct DQB locus, which we propose to designate BoLA-DQB5, (2) DQB5 constitutes an ancient DQB locus which diverged from a common ancestor gene prior to the duplication resulting in DQB1 and DQB2, (3) DQB5 is associated with haplotypes which contain DQA5 and a duplicated DQ region.