Building neurophenomics in zebrafish: Effects of prior testing stress and test batteries
Building neurophenomics in zebrafish: Effects of prior testing stress and test batteries
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DOI：
10.1016/j.bbr.2016.05.005
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发表时间：
2016-09
影响因子：
2.7
通讯作者：
Cai Song;Lei Yang;Jiajia Wang;Peirong Chen;Shaoming Li;Yingcong Liu;Michael Nguyen;Aleksandra A. Kaluyeva;Evan J. Kyzar;Siddharth Gaikwad;A. Kalueff
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文献类型：
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作者：
Cai Song;Lei Yang;Jiajia Wang;Peirong Chen;Shaoming Li;Yingcong Liu;Michael Nguyen;Aleksandra A. Kaluyeva;Evan J. Kyzar;Siddharth Gaikwad;A. Kalueff
斑马鱼（Danio rerio）是一种很有前途的神经表型组学模型生物，神经表型组学是神经科学的一个新领域，将神经表型与各种遗传和环境因素联系起来。然而，先前的实验操作对不同行为范式中斑马鱼表现的影响仍不清楚。在这里，我们在两种常用模型——新型坦克（NTT）和明暗盒（LDB）测试——中研究了选定的压力程序和测试电池对成年斑马鱼焦虑样行为的影响。虽然在基线（对照）条件下的两项测试中，远交短鳍野生型（WT）和突变“粉红色”萤火虫之间没有明显的行为差异，但在这些测试中，急性严重压力源（30分钟的汽车运输）检测到突变鱼的焦虑样行为显着降低。相比之下，WT 斑马鱼在轻度应激源（5 分钟 40 Wt 光）暴露后没有表现出明显的 NTT 或 LDB 反应，也显示 NTT 和 LDB 电池相继立即运行或间隔 1 天运行没有差异。总的来说，这些发现表明，斑马鱼对测试电池效应的敏感度可能相对较低（例如，与其他流行物种相比，如啮齿动物），并表明可能需要更强的应激源（以补充低至中度应激的水生屏幕），以更好地揭示斑马鱼测定中的表型差异。这项研究强调了斑马鱼模型在神经表型研究中的价值，表明在斑马鱼行为测定中使用更多测试电池和更广泛的预测试压力源的潜力。
The zebrafish (Danio rerio) is a promising model organism for neurophenomics – a new field of neuroscience linking neural phenotypes to various genetic and environmental factors. However, the effects of prior experimental manipulations on zebrafish performance in different behavioral paradigms remain unclear. Here, we examine the influence of selected stressful procedures and test batteries on adult zebrafish anxiety-like behaviors in two commonly used models – the novel tank (NTT) and the light-dark box (LDB) tests. While no overt behavioral differences between outbred short-fin wild-type (WT) and mutant ‘pink’ glowfish were seen in both tests under baseline (control) conditions, an acute severe stressor (a 30-min car transportation) detected significantly lower mutant fish anxiety-like behavior in these tests. In contrast, WT zebrafish showed no overt NTT or LDB responses following a mild stressor (5-min 40-Wt light) exposure, also showing no differences in batteries of NTT and LDB run immediately one after another, or with a 1-day interval. Collectively, these findings suggest that zebrafish may be relatively less sensitive (e.g., than other popular species, such as rodents) to the test battery effect, and show that stronger stressors may be needed (to complement low-to-moderate stress aquatic screens) to better reveal phenotypical variance in zebrafish assays. Strengthening the value of zebrafish models in neurophenotyping research, this study indicates the potential of using more test batteries and a wider spectrum of pre-test stressors in zebrafish behavioral assays.