Increased Protein and mRNA Expression of Resistin After Dexamethasone Administration
Increased Protein and mRNA Expression of Resistin After Dexamethasone Administration
复制标题
地塞米松给药后抵抗素蛋白和 mRNA 表达增加
DOI：
10.1055/s-0034-1383651
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发表时间：
2015
影响因子：
2.2
通讯作者：
Kunugi H
中科院分区：
文献类型：
--
作者：
Sasayama D;Hori H;Nakamura S;Yamamoto N;Hattori K;Teraishi T;Ota M;Kunugi H
地塞米松等合成糖皮质激素广泛用于治疗各种炎症和自身免疫性疾病，但它们可能会引起包括高血糖在内的不良事件。为了阐明地塞米松的作用和作用机制，我们检查了地塞米松引起的基因表达水平的变化。对24名身体健康受试者在基线和地塞米松给药后收集的全血进行了微阵列分析。发现地塞米松给药后抵抗素 mRNA 的表达水平显着增加。在 12 名受试者的单独样本中，我们检查了血浆抵抗素蛋白水平，发现服用地塞米松后血浆抵抗素蛋白水平有所增加。此外，携带RETN单核苷酸多态性rs3219175 A等位基因的个体的血浆抵抗素mRNA和蛋白质水平显着高于不携带该等位基因的个体。基因型和地塞米松给药之间没有显着的相互作用。血浆皮质醇和抵抗素水平之间没有发现显着相关性。与之前的研究一致，RETNrs3219175 的基因型是抵抗素水平的重要决定因素。本研究表明，无论 rs3219175 基因型如何，口服地塞米松都会增加抵抗素的蛋白质和 mRNA 水平。
Synthetic glucocorticoids such as dexamethasone are widely used to treat a variety of inflammatory and autoimmune conditions, but they may induce adverse events including hyperglycemia. To shed light on the effect and action mechanism of dexamethasone, we examined the alterations of gene expression levels caused by dexamethasone.Microarray analysis was performed on whole blood collected from 24 physically healthy subjects at baseline and after dexamethasone administration. The expression levels of resistin mRNA were found to be significantly increased after the dexamethasone administration. In a separate sample of 12 subjects, we examined plasma resistin protein levels and found that they were increased after dexamethasone administration. Furthermore, the plasma mRNA and protein levels of resistin were significantly higher in individuals who carried the A allele ofRETNsingle nucleotide polymorphism rs3219175 than in those who did not carry the allele. There was no significant interaction between the genotype and dexamethasone administration. No significant correlation was found between plasma levels of cortisol and resistin. Consistent with previous studies, the genotype ofRETNrs3219175 was a strong determinant of resistin levels. The present study showed that oral administration of dexamethasone increases the protein and mRNA levels of resistin irrespective of the rs3219175 genotype.