In vitro analysis of PNIPAAm-PEG, a novel, injectable scaffold for spinal cord repair.
In vitro analysis of PNIPAAm-PEG, a novel, injectable scaffold for spinal cord repair.
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DOI：
10.1016/j.actbio.2008.10.008
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发表时间：
2009-05
影响因子：
9.7
通讯作者：
Lowman, Anthony
中科院分区：
文献类型：
--
作者：
Comolli, Noelle;Neuhuber, Birgit;Fischer, Itzhak;Lowman, Anthony
神经组织工程与其他治疗策略相结合是治疗不同中枢神经系统疾病和损伤的新趋势。我们建议使用聚(N-异丙基丙烯酰胺)-聚乙二醇共聚物(PNIPAAm-PEG)作为一种微创、可注射的支架平台来修复脊髓损伤(SCI)。该支架允许细胞附着，提供机械支持和神经营养因子的持续释放。为了使用PNIPAAm-PEG作为治疗脊髓损伤的可注射支架，它必须在生理条件下保持其质量和体积不变。为了在损伤部位提供机械支持，工程支架与天然神经元组织的压缩模数匹配也是至关重要的。本研究的重点是研究支架释放生物活性神经营养因子的能力，并将材料特性与天然神经元组织的材料特性进行匹配。我们发现，BDNF和NT-3的释放持续了长达四周，两种神经营养因子的释放都表现出最小的爆发。4周后证实释放的NT-3和BDNF具有生物活性。此外，我们的结果表明，PNIPAAm-PEG支架可以设计成与自然神经元组织所需的机械性能相匹配，压缩模数在3-5kpa范围内。该支架还与骨髓基质细胞兼容，使其存活和附着长达31天。这些结果表明，PNIPAAm-PEG是一种很有前途的治疗脊髓损伤的多功能支架。
Nervous tissue engineering in combination with other therapeutic strategies is an emerging trend for the treatment of different CNS disorders and injuries. We propose to use poly (N-isopropylacrylamide)-co-poly (ethylene glycol) (PNIPAAm-PEG) as a minimally invasive, injectable scaffold platform for the repair of spinal cord injury (SCI). The scaffold allows cell attachment, provides mechanical support and a sustained release of neurotrophins. In order to use PNIPAAm-PEG as an injectable scaffold for treatment of SCI it must maintain its mass and volume over time in physiological conditions. To provide mechanical support at the injury site, it is also critical that the engineered scaffold matches the compressive modulus of the native neuronal tissue. This study focused on studying the ability of the scaffold to release bioactive neurotrophins and on matching the material properties to those of the native neuronal tissue. We found that the release of both BDNF and NT-3 was sustained for up to four weeks, with a minimal burst exhibited for both neurotrophins. The bioactivity of the released NT-3 and BDNF was confirmed after four weeks. In addition, our results show that the PNIPAAm-PEG scaffold can be designed to match the desired mechanical properties of the native neuronal tissue, with a compressive modulus in the 3–5kPa range. The scaffold was also compatible with bone marrow stromal cells, allowing their survival and attachment for up to 31 days. These results indicate that PNIPAAm-PEG is a promising multifunctional scaffold for the treatment of SCI.
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