DPY-27 - A CHROMOSOME CONDENSATION PROTEIN HOMOLOG THAT REGULATES C. ELEGANS DOSAGE COMPENSATION THROUGH ASSOCIATION WITH THE X-CHROMOSOME
DPY-27 - A CHROMOSOME CONDENSATION PROTEIN HOMOLOG THAT REGULATES C. ELEGANS DOSAGE COMPENSATION THROUGH ASSOCIATION WITH THE X-CHROMOSOME
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DOI：
10.1016/0092-8674(94)90255-0
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发表时间：
1994-11-04
期刊：
影响因子：
64.5
通讯作者：
MEYER, BJ
中科院分区：
文献类型：
--
作者：
CHUANG, PT;ALBERTSON, DG;MEYER, BJ
dpy - 27是一个必需的剂量补偿基因，其作用是降低雌雄同体的两条X染色体的表达。DPY - 27蛋白特异地定位于野生型XX胚胎的X染色体上，但在雄性（XO）胚胎的细胞核中呈弥散分布。在xol - 1突变的XO胚胎中，其激活了XX剂量补偿模式，并因X染色体转录水平过低而死亡，DPY - 27定位于X染色体。因此，剂量补偿过程的性别特异性在DPY - 27定位于X染色体这一步骤受到调控。DPY - 27与在体外非洲爪蟾染色体组装和结构维持以及在体内酵母染色体分离所必需的蛋白质具有显著的相似性。这些发现表明基因表达的整体调控与高级染色体结构之间存在联系。我们提出DPY - 27通过以一种导致X染色体表达普遍降低的方式浓缩X染色体的染色质结构来实现剂量补偿。
dpy-27 is an essential dosage compensation gene that acts to reduce expression of both hermaphrodite X chromosomes. The DPY-27 protein becomes specifically localized to the X chromosomes of wild-type XX embryos, but remains diffusely distributed throughout the nuclei of male (XO) embryos. In xol-1 mutant XO embryos that activate the XX mode of dosage compensation and die from inappropriately low X chromosome transcript levels, DPY-27 becomes localized to X. Therefore, sex specificity of the dosage compensation process is regulated at the step of DPY-27 X chromosome localization. DPY-27 exhibits striking similarity to proteins required for assembly and structural maintenance of Xenopus chromosomes in vitro and for segregation of yeast chromosomes in vivo. These findings suggest a link between global regulation of gene expression and higher order chromosome structure. We propose that DPY-27 implements dosage compensation by condensing the chromatin structure of X in a manner that causes general reduction of X chromosome expression.