Effect of Geographical Location and Type of Extract on Total Phenol/Flavon Contents and Antioxidant Activity of Different Fruits Extracts of Withania somnifera
Effect of Geographical Location and Type of Extract on Total Phenol/Flavon Contents and Antioxidant Activity of Different Fruits Extracts of Withania somnifera
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DOI：
10.2174/1570163815666180807100456
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发表时间：
2020-01-01
影响因子：
--
通讯作者：
Semalty, Mona
中科院分区：
文献类型：
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作者：
Adhikari, Lokesh;Kotiyal, Rupali;Semalty, Mona
工作背景：睡莲（茄科）是一种被广泛研究的药用植物，由于其广泛的药用特性，也被称为印度人参。植物的含量和活性可能会有所不同，这取决于植物的栖息地和部分以及用于提取的溶剂。本研究对西葫芦甲醇提取物及其提取物（乙酸乙酯、丁醇和水）进行了比较化学分析和体外抗氧化活性研究。方法：在本研究中，从两个不同的地理位置（北阿坎德和拉贾斯坦邦）收集睡茄果实。制备了不同的果实提取物，并研究了总酚和总黄酮含量。采用DPPH自由基清除法和过氧化氢清除法测定其体外抗氧化活性。somnifera北阿坎德和W.总酚含量（TPC）以拉贾斯坦邦最高。In W.北阿坎德的乙酸乙酯提取物中总黄酮含量最高，而西洋参的乙酸乙酯提取物中总黄酮含量最高。结果表明，催眠草拉贾斯坦邦甲醇提取物中黄酮类化合物含量最高。W.甲醇提取物Somnifera北阿坎德的清除自由基能力最强，而W.抗氧化活性以甲醇提取物最高，其次为丁醇提取物，水提取物，乙酸乙酯提取物最低。在抗氧化活性的过氧化氢清除试验中，水提物的抗氧化活性最高。而北阿坎德邦（北阿坎德）、W.结论：不同地理位置对水杨梅中活性成分的含量及抗氧化活性有重要影响。嗜睡。
Background: Withania somnifera (family solanaceae) is a well-investigated medicinal plant which is also called Indian ginseng due to its wide spectrum of medicinal properties. The contents and activity of the plant may vary depending on the habitat and part of the plant and the solvent used for extraction. The present study deals with the comparative chemical analysis and in vitro antioxidant activity of methanolic fruits extracts and its subtractions (in ethyl acetate, butanol and water) of W. somnifera collected from two different geographical locations.Methods: In the present study, Withania somnifera fruits were collected from two different geographical locations (Uttarakhand and Rajasthan). The different fruit extracts were prepared and studied for total phenolic contents and total flavone contents. The in vitro antioxidant activity was assessed by DPPH free radical scavenging assay and peroxide scavenging assay.Results: Methanol extract of W. somnifera Uttarakhand and ethyl acetate subfraction of W. somnifera Rajasthan showed the highest amount of Total Phenolic Contents (TPC). In W. somnifera Uttarakhand, ethyl acetate extract showed the highest amount of Total flavonoids while in W. somnifera Rajasthan, methanol extract was found to be the richest in flavonoids. Methanolic extract of W. somnifera Uttarakhand showed the highest free radical scavenging activity while in W. somnifera Rajasthan, the highest antioxidant activity was shown by the methanolic extract followed by butanolic extract, water extract and then ethyl acetate. In the peroxide scavenging assay of antioxidant activity, water extract of W. somnifera Uttarakhand showed the highest activity, while in W. somnifera Rajasthan, ethyl acetate extract showed highest scavenging activity.Conclusion: It was concluded that the geographical location exerts a vital effect on the presence of active constituents and also on the antioxidant potential of W. somnifera.