A mechanism for substrate-Induced formation of 6-hydroxyflavin mononucleotide catalyzed by C30A trimethylamine dehydrogenase.
A mechanism for substrate-Induced formation of 6-hydroxyflavin mononucleotide catalyzed by C30A trimethylamine dehydrogenase.
复制标题
C30A 三甲胺脱氢酶催化底物诱导形成 6-羟基黄素单核苷酸的机制。
DOI：
10.1016/j.bmcl.2003.07.032
复制
发表时间：
2003
影响因子：
2.7
通讯作者：
Silverman,RichardB
中科院分区：
文献类型：
--
作者：
Lu,Xingliang;Nikolic,Dejan;Mitchell,DeannaJ;vanBreemen,RichardB;Mersfelder,JohnA;Hille,Russ;Silverman,RichardB
Experiments are described to determine the origin of the 6-hydroxyl group of 6-hydroxyFMN produced by the substrate-induced transformation of FMN in the C30A mutant of trimethylamine dehydrogenase. The conversion of FMN to 6-hydroxyFMN is carried out in the presence of H218O and18O2, and the results clearly show that the 6-hydroxyl group is derived from molecular oxygen and not from water.
