Substrate specificity of FUT8 and chemoenzymatic synthesis of core-fucosylated asymmetric N-glycans.
Substrate specificity of FUT8 and chemoenzymatic synthesis of core-fucosylated asymmetric N-glycans.
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DOI：
10.1039/c6ob00586a
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发表时间：
2016-04-26
影响因子：
3.2
通讯作者：
Wang PG
中科院分区：
文献类型：
--
作者：
Calderon AD;Liu Y;Li X;Wang X;Chen X;Li L;Wang PG
Substrate specificity studies of human FUT8 using 77 structurally-defined N-glycans as acceptors showed a strict requirement towards the α1,3-mannose branch, but a great promiscuity towards the α1,6-mannose branch. Accordingly, a chemoenzymatic strategy was developed for efficient synthesis of core-fucosylated asymmetric N-glycans. Based on substrate specificity of human FUT8, an efficient strategy was developed for the synthesis of core-fucosylated asymmetric N-glycans.
