SCHWANN-CELL MIGRATION THROUGH FREEZE-KILLED PERIPHERAL-NERVE GRAFTS WITHOUT ACCOMPANYING AXONS
SCHWANN-CELL MIGRATION THROUGH FREEZE-KILLED PERIPHERAL-NERVE GRAFTS WITHOUT ACCOMPANYING AXONS
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DOI：
10.1007/bf00294445
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发表时间：
1991-01-01
影响因子：
12.7
通讯作者：
TURMAINE, M
中科院分区：
文献类型：
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作者：
ANDERSON, PN;NADIM, W;TURMAINE, M
在成年近交系Fischer大鼠上，将冷冻干燥的胫神经移植物分别与切断的胫神经近端或远端吻合。在移植到近端残端的情况下，胫神经被结扎三次，离脊髓最远端的结扎距离吻合口1厘米。因此，在这两种类型的实验中，雪旺细胞可以自由进入最初的无细胞移植物，而不伴随轴突。术后17天~12周对移植物进行检查。用S-100蛋白免疫荧光检测雪旺细胞的迁移距离，用电子显微镜观察侵入移植物的细胞的形态。雪旺细胞在近端和远端残端的迁移相似。迁移的雪旺细胞形成类似邦纳带的柱状结构。它们主要是在被杀死的神经纤维留下的预先存在的基板管内发现的，但不是唯一的。一些雪旺细胞即使缺乏轴突接触，也会分泌一层薄薄的、片状的基底膜。雪旺细胞柱被成纤维细胞的突起部分分隔。髓鞘和其他碎片在移植物中被大量雪旺细胞穿透的部分清除得最快。雪旺细胞穿透移植物的最大距离为8.5 mm，于术后6~8周达到。这大约是雪旺细胞通过类似移植物伴随再生轴突迁移的最大距离的一半。本文讨论了雪旺细胞在无轴突的情况下较短距离迁移的原因，以及这些结果对解释使用无细胞移植物进行轴突再生实验的意义。
Freeze-dried tibial nerve grafts were anastomosed to either the proximal stump or the distal stump of severed tibial nerves in adult inbred Fischer rats. In the case of grafts attached to the proximal stump the tibial nerve was ligated three times, the most distal ligature from the spinal cord being 1 cm from the site of anastomosis. In both types of experiment Schwann cells were, therefore, free to enter the initially acellular grafts without accompanying axons. The grafts were examined 17 days to 12 weeks after operation. Immunofluorescence for S-100 protein was used to evaluate the distance migrated by the Schwann cells and electron microscopy was used to examine the morphology of the cells which invaded the grafts. Schwann cell migration was similar from the proximal and distal stumps. The migrating Schwann cells formed columns which resembled bands of Bungner. They were found mainly, but not exclusively, inside the pre-existing basal lamina tubes left behind by the killed nerve fibres. Some Schwann cells secreted a thin, patchy basal lamina even though they lacked axonal contact. Schwann cell columns became partially compartmentalized by fibroblast processes. Myelin and other debris were removed most rapidly in those parts of the grafts penetrated by large numbers of Schwann cells. The maximum distance the Schwann cells penetrated into the grafts was 8.5 mm and this was achieved by 6 to 8 weeks after operation. This is about half the maximum distance migrated by Schwann cells accompanying regenerating axons through similar grafts. The reasons why Schwann cells migrate shorter distances without axons and the significance of these results for the interpretation of axonal regeneration experiments using acellular grafts are discussed.