Aptasensors for rapid detection of Escherichia coli O157:H7 and Salmonella typhimurium.
Aptasensors for rapid detection of Escherichia coli O157:H7 and Salmonella typhimurium.
复制标题
用于快速检测大肠杆菌 O157:H7 和鼠伤寒沙门氏菌的适配体传感器
DOI：
10.1186/1556-276x-7-658
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发表时间：
2012-11-29
影响因子：
--
通讯作者：
Lu JX
中科院分区：
文献类型：
--
作者：
Wu WH;Li M;Wang Y;Ouyang HX;Wang L;Li CX;Cao YC;Meng QH;Lu JX
在此，我们报道了基于无标记适配子和金纳米颗粒(AuNPs)的适体生物传感器的发展，用于检测大肠杆菌O157：H7和鼠伤寒沙门氏菌。目标细菌结合适配子吸附在未经修饰的AuNPs表面以捕获目标细菌，检测是通过目标细菌诱导适配子在高盐条件下聚集成红色到紫色的颜色变化来完成的。通过使用抗E。ColiO157：H7适配子和抗S。我们开发了一种方便、快速的方法，可以选择性地检测细菌，而不需要专门的仪器和细胞裂解等前处理步骤。该适体传感器可在20min或更短时间内检测到低至105个菌落形成单位(CFU)/ml的目标菌，其特异度为100%。这一新方法在细菌的快速检测中具有很大的应用潜力。
Herein we reported the development of aptamer-based biosensors (aptasensors) based on label-free aptamers and gold nanoparticles (AuNPs) for detection of Escherichia coli (E. coli) O157:H7 and Salmonella typhimurium. Target bacteria binding aptamers are adsorbed on the surface of unmodified AuNPs to capture target bacteria, and the detection was accomplished by target bacteria-induced aggregation of the aptasensor which is associated as red-to-purple color change upon high-salt conditions. By employing anti-E. coli O157:H7 aptamer and anti-S. typhimurium aptamer, we developed a convenient and rapid approach that could selectively detect bacteria without specialized instrumentation and pretreatment steps such as cell lysis. The aptasensor could detect as low as 105colony-forming units (CFU)/ml target bacteria within 20 min or less and its specificity was 100%. This novel method has a great potential application in rapid detection of bacteria in the near future.