ACTIVATION BY SACCHARIDES OF A CATION-SELECTIVE PATHWAY ON CANINE LINGUAL EPITHELIUM
ACTIVATION BY SACCHARIDES OF A CATION-SELECTIVE PATHWAY ON CANINE LINGUAL EPITHELIUM
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DOI：
10.1152/ajpregu.1989.256.2.r394
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发表时间：
1989-02-01
影响因子：
--
通讯作者：
ROBB, R
中科院分区：
文献类型：
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作者：
SIMON, SA;LABARCA, P;ROBB, R
Ussing小室中分离的犬舌上皮对D-葡萄糖和果糖的反应揭示了与味觉传导相关的事件。利用同位素通量研究，结合离子替代、药理和电压钳测量，发现D-葡萄糖刺激离子转运的原因是阳离子选择性途径中阳离子内流增加。这种阳离子的流入被0.1 mM的阿米洛利完全抑制。阿米洛利也能抑制0.05M KCl中果糖和0.05MrbcL中D-葡萄糖对转运的刺激，表明糖刺激的进入途径既不是己糖所特有的，也不是Na所特有的。糖对犬舌上皮细胞的刺激似乎不受细胞内3‘，5’-环一磷酸、鸟苷3‘’，5‘’-环一磷酸或钙水平增加的调节。在糖的刺激下进入味觉细胞的钠通过位于浆膜膜上的哇宾可抑制的Na+-K+-三磷酸腺苷酶存在于味觉细胞中。
Responses of isolated canine lingual epithelium in an ussing chamber to D-glucose and fructose reveal events associated with taste transduction. With the use of isotopic flux studies, together with ion substitution and pharmacological and voltage clamp measurements, it was found that the stimulation of ion transport by D-glucose arises from an increase in the influx of cations through a cation-selective pathway. This influx of cations is completely inhibited by 0.1 mM amiloride. The stimulation of transport by fructose in 0.05 M KCl and by D-glucose in 0.05 M RbCl was also inhibited by amiloride, demonstrating that the saccharide-stimulated entry pathway was specific for neither hexose sugars nor for Na. Saccharide stimulation of canine lingual epithelia does not appear to be modulated by increases in intracellular levels of adenosine 3'',5''-cyclic monophosphate, guanosine 3'',5''-cyclic monophosphate, or Ca. The Na that enters taste cells on saccharide stimulation exists them via the ouabin inhibitable Na+-K+-adenosinetriphosphatase located in the serosal membranes.