Swine testis cells contain functional heparan sulfate but are defective in entry of herpes simplex virus.
Swine testis cells contain functional heparan sulfate but are defective in entry of herpes simplex virus.
复制标题
猪睾丸细胞含有功能性硫酸乙酰肝素，但在单纯疱疹病毒的进入方面存在缺陷。
DOI：
10.1128/jvi.68.9.5667-5676.1994
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发表时间：
1994
影响因子：
5.4
通讯作者：
Fuller,AO
中科院分区：
文献类型：
--
作者：
Subramanian,G;McClain,DS;Perez,A;Fuller,AO
单纯疱疹病毒（HSV）进入并感染大多数培养细胞。我们已经发现，猪睾丸细胞（ST）产生的感染性HSV-1的产量高达四个数量级低于人胚肺（HEL）和HEp-2细胞，因为在病毒进入的缺陷。对于ST细胞，病毒结合减少，不能检测到来自输入病毒的DNA，并且不能合成病毒蛋白。暴露于HSV后ST细胞的聚乙二醇处理允许病毒进入、蛋白质合成和生产性感染。绕过正常进入过程的病毒基因组DNA的转染从ST、HEL和HEp-2细胞产生相似的感染性病毒产量。因此，所有三种细胞系都可以支持HSV复制周期。生化分析和氯酸钠处理的硫酸化抑制表明，ST细胞含有硫酸乙酰肝素（HS）的数量和类型类似的高度敏感的细胞。氯酸钠处理的HEL和ST细胞的HSV感染表明易感HEp-2和HEL细胞上存在第二种非HS受体，其在易感性差的ST细胞上缺失或不起作用。我们得出结论，ST细胞在HSV进入中有缺陷，含有功能性HS，但缺乏有效HSV-1进入所需的功能性非HS受体。此外，ST细胞提供了一种新的资源，其可用于鉴定、分离和表征HSV非HS受体及其在这种重要的人类病原体的进入和向性中的作用。
Herpes simplex virus (HSV) enters and infects most cultured cells. We have found that swine testis cells (ST) produce yields of infectious HSV-1 up to four orders of magnitude lower than those of human embryonic lung (HEL) and HEp-2 cells because of a defect in virus entry. For ST cells, virus binding is reduced, DNA from input virus cannot be detected, and virus proteins are not synthesized. Polyethylene glycol treatment of ST cells after exposure to HSV allows viral entry, protein synthesis, and productive infection. Transfection of viral genomic DNA that bypasses the normal entry process produces similar yields of infectious virus from ST, HEL, and HEp-2 cells. Therefore, all three cell lines can support the HSV replicative cycle. Biochemical analyses and inhibition of sulfation by sodium chlorate treatment show that ST cells contain amounts and types of heparan sulfate (HS) similar to those of highly susceptible cells. HSV infection of sodium chlorate-treated HEL and ST cells indicates the presence of a second, non-HS receptor(s) on susceptible HEp-2 and HEL cells that is missing, or not functional, on poorly susceptible ST cells. We conclude that ST cells are defective in HSV entry, contain functional HS, but lack a functional non-HS receptor(s) required for efficient HSV-1 entry. Further, ST cells provide a novel resource that can be used to identify, isolate, and characterize an HSV non-HS receptor(s) and its role in the entry and tropism of this important human pathogen.