Alteration of Mucins in Rat Gastric Mucosa Healing from Acetic Acid-induced Ulcer: An Immunohistochemical Study Using Monoclonal Antibodies against Gastric Mucins
Alteration of Mucins in Rat Gastric Mucosa Healing from Acetic Acid-induced Ulcer: An Immunohistochemical Study Using Monoclonal Antibodies against Gastric Mucins
复制标题
醋酸诱导溃疡大鼠胃粘膜愈合中粘蛋白的变化：使用抗胃粘蛋白单克隆抗体的免疫组织化学研究
DOI：
10.2220/biomedres.21.95
复制
发表时间：
2000
影响因子：
1.2
通讯作者：
K. Hotta
中科院分区：
文献类型：
--
作者：
H. Hayashida;K. Ishihara;M. Kurihara;K. Saigenji;K. Hotta
我们研究了粘蛋白的变化，在大鼠胃粘膜乙酸损伤的免疫组化方法，使用三个不同的单克隆抗体（MAbs）对胃粘蛋白：RGM 21，RGM 26和HIKIOS 3，每一个反应的粘蛋白分子位于特定区域和层的大鼠胃粘膜。在8周龄雄性Wistar大鼠中制备乙酸诱导的溃疡，并在酸损伤后10、20和30天处死动物。即使在第10天，RGM 26阳性的粘液细胞，它只能识别正常粘膜胃窦区的表面粘液细胞，出现在再生上皮。20-30天时，不仅这些上皮的远端侧，而且近端侧都被这种MAb强染色的细胞占据。与识别正常粘膜体表面上皮细胞的RGM 21的反应在再生上皮中减少。HlKlO_（83）染色显示，溃疡愈合早期，腺体粘液细胞增多，20-30天染色范围扩大至再生粘膜底部。这些结果表明，在创伤愈合过程中，胃窦型粘蛋白在再生上皮中有不同的表达。
We examined the alteration of mucins in the rat gastric mucosa injured with acetic acid by means of an immunohistochemical method using three distinct monoclonal antibodies (MAbs) against the gastric mucins: RGM2l, RGM26 and HIKIOS3, each reacting with the mucin molecule located in the specific region and layer of the rat gastric mucosa. An acetic acid induced-ulcer was prepared in eight-Week-old male Wistar rats and the animals were killed at 10, 20 and 30 days after the acid insult. Even at 10 days, mucous cells positive to RGM26, which can only recognize the surface mucous cells of antral region in the normal mucosa, emerged at the regenerating epithelia. At 20-30 days, not onlythe distal side, but also the proximal side of these epithelia were occupied with the cells strongly stained with this MAb. The reaction with RGM2l, which recognizes the surface epithelial cells of normal corpus mucosa, reduced in the regenerating epithelia. HlKlO83 staining indicating the gland mucous cells increased around the ulcer area at earlier stage of healing, and the staining area was expanded to the bottom of the regenerating mucosae at 20-30 days. These results indicate a distinguished expression of the antral—type mucins in the regenerating epithelia during the healing process of