Functional analyses of RNA structures shared between the internal ribosome entry sites of hepatitis C virus and the picornavirus porcine teschovirus 1 Talfan
Functional analyses of RNA structures shared between the internal ribosome entry sites of hepatitis C virus and the picornavirus porcine teschovirus 1 Talfan
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DOI：
10.1128/jvi.80.3.1271-1279.2006
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发表时间：
2006-02-01
影响因子：
5.4
通讯作者：
Belsham, GJ
中科院分区：
文献类型：
--
作者：
Chard, LS;Kaku, Y;Belsham, GJ
小核糖体病毒科成员之一的猪圆糖病毒 I (PTV-1) 的内部核糖体进入位点 (IRES) 与其他已明确表征的小核糖体病毒 IRES 元件截然不同，但它与黄病毒科成员丙型肝炎病毒 (HCV) 的 IRES 功能相似。特别是，eIF4A 的显性失活突变体形式不会抑制 PTV-1 IRES 的活性。此外，PTV-1 和 HCV IRES 序列之间存在高水平（约 50%）的同一性。已经导出了整个 PTV-1 IRES 的二级结构模型，其中包括一个假结。模型内特定特征的验证已通过诱变和功能测定来实现。 PTV-1 和 HCV IRES 要素之间的差异和相似之处应有助于定义此类 IRES 的关键特征。
The internal ribosome entry site (IRES) of porcine teschovirus I (PTV-1), a member of the Picornaviridae family, is quite distinct from other well-characterized picornavirus IRES elements, but it displays functional similarities to the IRES from hepatitis C virus (HCV), a member of the Flaviviridae family. In particular, a dominant negative mutant form of eIF4A does not inhibit the activity of the PTV-1 IRES. Furthermore, there is a high level (ca. 50%) of identity between the PTV-1 and HCV IRES sequences. A secondary-structure model of the whole PTV-1 IRES has been derived which includes a pseudoknot. Validation of specific features within the model has been achieved by mutagenesis and functional assays. The differences and similarities between the PTV-1 and HCV IRES elements should assist in defining the critical features of this type of IRES.